The Institute of Microbiology and Biotechnology, 1 Academiei Str., 2028, Chisinau, Moldova.
State University "Dmitrie Cantemir", 3/2 Academiei Str, 2028, Chisinau, Moldova.
Environ Sci Pollut Res Int. 2020 Sep;27(25):31793-31811. doi: 10.1007/s11356-020-09447-z. Epub 2020 Jun 5.
Metal accumulation by Spirulina platensis from synthetic effluents with the following chemical composition: Cr/Fe, Cr/Fe/Ni, Cr/Fe/Ni/Zn, and Cr/Fe/Ni/Zn/Cu during repeated cultivation cycle was investigated. Metal ions in different concentrations were added to the culture medium at the exponential and stationary phases of biomass growth and their uptake by biomass was traced using neutron activation analysis. The effect of metal ions on biomass and main biochemical components (proteins, carbohydrates, lipids, phycobilins, and β-carotene) was monitored. S. platensis keeps high metal accumulation capacity during 2-3 cultivation cycles, while the metal ions were added in the stationary phase of its growth. By adding metals in the exponential phase of growth in the following concentrations: 10 mg/L of chromium (VI), 5 mg/L of iron, 2 mg/L of zinc, nickel, and copper, Spirulina platensis acted as renewable accumulator only in Cr/Fe system. It maintained the accumulation capacity during three cultivation cycles when exposed to lower concentrations of metal ions. Its ability to accumulate metal ions during several cultivation cycles was ensured by the maintenance of the optimal level of proteins and lipid in biomass.
Cr/Fe、Cr/Fe/Ni、Cr/Fe/Ni/Zn 和 Cr/Fe/Ni/Zn/Cu。在生物量生长的指数和稳定阶段，将不同浓度的金属离子添加到培养基中，并使用中子激活分析跟踪生物质对金属离子的吸收。监测金属离子对生物质和主要生化成分（蛋白质、碳水化合物、脂质、藻胆蛋白和β-胡萝卜素）的影响。螺旋藻在 2-3 个培养周期内保持高金属积累能力，而金属离子是在其生长的稳定阶段添加的。通过在生长的指数阶段添加以下浓度的金属：10mg/L 的六价铬、5mg/L 的铁、2mg/L 的锌、镍和铜，螺旋藻仅在 Cr/Fe 体系中表现为可再生积累器。当暴露于较低浓度的金属离子时，它在三个培养周期内保持了积累能力。它在几个培养周期中积累金属离子的能力是通过维持生物质中蛋白质和脂质的最佳水平来保证的。
