Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland.
Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100, Pulawy, Poland.
Carbohydr Res. 2020 Jul;493:108036. doi: 10.1016/j.carres.2020.108036. Epub 2020 May 21.
The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas veronii bv. sobria strain Pt393, which is pathogenic to the rainbow trout (Oncorhynchus mykiss), after mild acid hydrolysis followed by GPC. The high-molecular-weight OPS fraction was studied with chemical methods, mass spectrometry, and H and C NMR spectroscopy techniques, including 2D H,H COSY, TOCSY, NOESY, H-detected heteronuclear H,C HSQC, and HMBC experiments. It was found that the O-specific polysaccharide was built of a tetrasaccharide repeating unit composed of α-GalpNAc, α-FucpNAc, β-QuipNAc, and α-Fucp4NAc (4-acetamido-4,6-dideoxy-d-galactose, tomosamine) residues. The following structure of the OPS of A. sobria strain Pt393 was established: →4)-α-d-GalpNAc-(1 → 3)-α-l-FucpNAc-(1 → 3)-β-d-QuipNAc-(1 → 3)-α-d-Fucp4NAc-(1→.
该 O-特异性多糖（OPS）是从致病性虹鳟鱼（Oncorhynchus mykiss）的气单胞菌 veronii bv. sobria 菌株 Pt393 的脂多糖中经温和酸水解和 GPC 分离得到的。采用化学方法、质谱和 1H 和 13C NMR 光谱技术，包括 2D 1H,1H COSY、TOCSY、NOESY、H 检测异核 1H,13C HSQC 和 HMBC 实验，对高分子量 OPS 部分进行了研究。结果表明，O-特异性多糖由四糖重复单元组成，由α-GalpNAc、α-FucpNAc、β-QuipNAc 和α-Fucp4NAc（4-乙酰氨基-4,6-二脱氧-d-半乳糖，tomosamine）残基组成。确定了 A. sobria 菌株 Pt393 的 OPS 的如下结构：→4）-α-d-GalpNAc-（1 → 3）-α-l-FucpNAc-（1 → 3）-β-d-QuipNAc-（1 → 3）-α-d-Fucp4NAc-（1→。
