Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
Carbohydr Res. 2021 Feb;500:108210. doi: 10.1016/j.carres.2020.108210. Epub 2020 Nov 25.
The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas veronii strain Bs8, which is pathogenic to common carp (Cyprinus carpio), after mild acid hydrolysis followed by gel-permeation chromatography. The high-molecular-mass OPS fraction was investigated using chemical methods, mass spectrometry, and H and C NMR spectroscopy techniques, including 2D homonuclear H,H TOCSY, DQF COSY, NOESY, and heteronuclear H-detected H,C HSQC, and HMBC experiments. The analysis revealed that the O-specific polysaccharide contains sugars with the galacto configuration of the ring and is composed of a disaccharide repeating unit with the following structure.
该 O-特异性多糖（OPS）是从致病性鲫鲤气单胞菌菌株 Bs8 的脂多糖中经弱酸水解后，通过凝胶渗透色谱分离得到的。采用化学方法、质谱和 H 和 C NMR 光谱技术，包括 2D 同核 H，H TOCSY、DQF COSY、NOESY 和异核 H 检测 H，C HSQC 和 HMBC 实验，对高分子质量 OPS 部分进行了研究。分析表明，O-特异性多糖含有具有半乳糖环构型的糖，由具有以下结构的二糖重复单元组成。
