School of Civil Engineering and Architecture, Northeast Electric Power University, Jilin, 132012, China.
School of Civil Engineering and Architecture, Northeast Electric Power University, Jilin, 132012, China.
Ecotoxicol Environ Saf. 2020 Sep 15;201:110739. doi: 10.1016/j.ecoenv.2020.110739. Epub 2020 Jun 4.
In the study, antibiotic resistance genes (ARGs) were examined in wastewater and sludge samples to explore the effect of cephalexin (CFX) on the spreading and removal of ARGs in the Expanded Granular Sludge Bed (EGSB) reactor treating antibiotics wastewater. The result showed that the addition of CFX in the wastewater affected the removal amount of β-lactam ARGs and other types ARGs. Besides, the addition of CFX in the wastewater had no obviously effect on total concentration of targeted ARGs in the sludge, but it was related to the accumulation of some typical ARGs. Based on gene cassette array libraries analysis, the diversity of gene cassettes carried by intI1 gene was increased by the addition of CFX in the wastewater. Furthermore, the co-occurrence patterns between ARGs and bacterial genus were also investigated. The results showed the CFX in the wastewater not only affected the number of potential host bacteria of ARGs, but also changed the types of potential host bacteria of ARGs. The correlation analysis of ARG in influent, effluent and sludge showed that, for blaCTX-M, sul2, qnrS and AmpC genes, their removal amount in EGSB reactor treating antibiotic wastewater system might be enhanced by reducing their concentration in the sludge.
在这项研究中，检测了废水和污泥样本中的抗生素耐药基因 (ARGs)，以探讨头孢氨苄 (CFX) 对处理抗生素废水的膨胀颗粒污泥床 (EGSB) 反应器中 ARGs 传播和去除的影响。结果表明，废水中添加 CFX 会影响 β-内酰胺类 ARGs 和其他类型 ARGs 的去除量。此外，废水中添加 CFX 对污泥中目标 ARGs 的总浓度没有明显影响，但与某些典型 ARGs 的积累有关。基于基因盒阵列文库分析，废水中添加 CFX 增加了携带 intI1 基因的基因盒的多样性。此外，还研究了 ARGs 与细菌属之间的共现模式。结果表明，废水中的 CFX 不仅影响了 ARGs 的潜在宿主细菌数量，还改变了 ARGs 的潜在宿主细菌类型。进水、出水和污泥中 ARG 的相关性分析表明，对于 blaCTX-M、sul2、qnrS 和 AmpC 基因，通过降低污泥中这些基因的浓度，可能会增强 EGSB 反应器处理抗生素废水系统中这些基因的去除量。
