College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China; Key Laboratory of Agro-Products Postharvest Handling, Ministry of Agriculture and Rural Affairs, Hangzhou, China; National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Zhejiang University, Hangzhou, China; Ningbo Research Institute, Zhejiang University, Ningbo, China.
Ultrason Sonochem. 2020 Nov;68:105184. doi: 10.1016/j.ultsonch.2020.105184. Epub 2020 May 27.
To enrich the properties of chitosan (CS) and improve its applicability, we prepared carboxymethyl-chitosan (CMCS) by carboxymethylation and developed a layer-by-layer assembly (LBL) by the ultrasonic nebulization-assisted technique. The degree of carboxymethylation was 42.20% and the structure of CMCS was characterized using Fourier transform infrared, nuclear magnetic resonance spectroscopy. The ultrasonic nebulization-assisted LBL (UNLBL) edible coating was developed based on an inner negatively CMCS charged layer and an external positively charged CS layer. As compared to conventional LBL (CLBL) assembly, the greater thickness, stronger mechanical properties and lower water vapor permeability were detected in UNLBL assembly. Through an untargeted metabolomic analysis and real-time quantitative PCR, it was evident that ultrasonic nebulization-assisted LBL assembly is a promising alternative for promoting phenylpropanoid metabolism in strawberries.
为了丰富壳聚糖 (CS) 的性质并提高其适用性，我们通过羧甲基化制备了羧甲基壳聚糖 (CMCS)，并通过超声雾化辅助技术开发了层层组装 (LBL)。羧甲基化程度为 42.20%，采用傅里叶变换红外光谱、核磁共振波谱对 CMCS 的结构进行了表征。基于带负电荷的 CMCS 内层和带正电荷的 CS 外层，开发了超声雾化辅助 LBL（UNLBL）可食用涂层。与传统的 LBL（CLBL）组装相比，UNLBL 组装的厚度更大、机械性能更强、水蒸气渗透率更低。通过非靶向代谢组学分析和实时定量 PCR 表明，超声雾化辅助 LBL 组装是促进草莓苯丙烷代谢的一种很有前途的替代方法。
