Wang Xiaoyu, Zhou Yuyang, Johnson Melissa, Milne Cameron, A Sigen, Li Yening, Wang Wenxin, Zhang Nan, Xu Qian
Charles Institute of Dermatology, School of Medicine, University College Dublin, D04 V1W8 Dublin, Ireland.
Centre of Micro/Nano Manufacturing Technology (MNMT-Dublin), School of Mechanical and Materials Engineering, University College Dublin, D04 KW52 Dublin, Ireland.
J Funct Biomater. 2023 Jan 12;14(1):43. doi: 10.3390/jfb14010043.
To explore the potential applicability of chitosan (CTS), we prepared aldehyde chitosan (CTS-CHO) with chitosan and sodium periodate via oxidation reaction and then a chitosan-based hydrophilic and antibacterial coating on the surface of poly (lactic acid) (PLA) film was developed and characterized. The oxidation degree was determined by Elemental analyser to be 12.53%, and a Fourier transform infrared spectroscopy was used to characterize the structure of CTS-CHO. It was evident that CTS-CHO is a biocompatible coating biomaterial with more than 80% cell viability obtained through the Live/Dead staining assay and the alamarBlue assay. The hydrophilic and antibacterial CTS-CHO coating on the PLA surface was prepared by ultrasonic atomization assisted LbL assembly technique due to Schiff's base reaction within and between layers. The CTS-CHO coating had better hydrophilicity and transparency, a more definite industrialization potential, and higher antibacterial activity at experimental concentrations than the CTS coating. All of the results demonstrated that the ultrasonic atomization-assisted LbL assembly CTS-CHO coating is a promising alternative for improving hydrophilicity and antibacterial activity on the PLA surface. The functional groups of CTS-CHO could react with active components with amino groups via dynamic Schiff's base reaction and provide the opportunity to create a drug releasing surface for biomedical applications.
为了探索壳聚糖（CTS）的潜在适用性，我们通过氧化反应，用壳聚糖和高碘酸钠制备了醛基壳聚糖（CTS-CHO），然后在聚乳酸（PLA）薄膜表面制备并表征了一种基于壳聚糖的亲水性抗菌涂层。通过元素分析仪测定氧化度为12.53%，并使用傅里叶变换红外光谱对CTS-CHO的结构进行表征。通过活/死染色法和alamarBlue法可知，CTS-CHO是一种生物相容性涂层生物材料，细胞活力超过80%。由于层内和层间的席夫碱反应，通过超声雾化辅助层层组装技术在PLA表面制备了亲水性抗菌CTS-CHO涂层。与CTS涂层相比，CTS-CHO涂层具有更好的亲水性和透明度，更明确的工业化潜力，并且在实验浓度下具有更高的抗菌活性。所有结果表明，超声雾化辅助层层组装CTS-CHO涂层是改善PLA表面亲水性和抗菌活性的一种有前景的替代方案。CTS-CHO的官能团可通过动态席夫碱反应与含氨基的活性成分反应，为生物医学应用创造药物释放表面提供了机会。
