Pugliese Daniela, Armuzzi Alessandro, Castri Federica, Benvenuto Roberta, Mangoni Antonella, Guidi Luisa, Gasbarrini Antonio, Rapaccini Gian Lodovico, Wolf Federica I, Trapani Valentina
UOC Medicina Interna e Gastroenterologia Columbus, Dipartimento di Medicina e Chirurgia Traslazionale, Fondazione Policlinico Universitario A. Gemelli IRCCS - Università Cattolica del Sacro Cuore, Rome, Italy.
UOC Anatomia Patologica, Dipartimento di Scienze della vita e Sanità pubblica, Fondazione Policlinico Universitario A. Gemelli IRCCS - Università Cattolica del Sacro Cuore, Rome, Italy.
Dig Liver Dis. 2020 Oct;52(10):1188-1194. doi: 10.1016/j.dld.2020.05.027. Epub 2020 Jun 3.
Inflammatory bowel disease (IBD) predisposes to colorectal cancer (CRC) with some specific features that distinguish it from sporadic CRC. Magnesium (Mg) homeostasis is severely compromised in IBD patients, which may affect both inflammation and tumor development. Efficient transcellular Mg transport in intestinal cells depends on the transient receptor potential melastatin (TRPM) channels type 6 and 7, but their expression has never been investigated in the context of IBD-related CRC.
We sought to study the expression pattern of TRPM6 and TRPM7 in CRC, and to compare IBD-related cases to sporadic cases.
TRPM6 and TRPM7 protein expression was evaluated by immunohistochemistry in surgical specimens from 16 IBD and 13 NON-IBD CRC patients.
TRPM7 expression was higher in tumor tissue than in the adjacent non-neoplastic tissue in both IBD and NON-IBD patients. Overall, adenocarcinomas showed a higher TRPM7 expression than adenomas. TRPM7 expression also positively correlated with tumor grade. Conversely, TRPM6 expression was higher in tumor tissues in both IBD and NON-IBD CRC, but it did not correlate with tumor stage or grade.
We report a possible participation of TRPM6 and 7 in both IBD-related and sporadic CRC and suggest that TRPM7 might serve as a marker of malignant transformation and lack of differentiation.
炎症性肠病（IBD）易患结直肠癌（CRC），其具有一些区别于散发性结直肠癌的特定特征。IBD患者的镁（Mg）稳态严重受损，这可能影响炎症和肿瘤发展。肠道细胞中高效的跨细胞镁转运依赖于瞬时受体电位褪黑素（TRPM）通道6型和7型，但它们在IBD相关结直肠癌中的表达从未被研究过。
我们试图研究TRPM6和TRPM7在结直肠癌中的表达模式，并将IBD相关病例与散发性病例进行比较。
通过免疫组织化学评估16例IBD和13例非IBD结直肠癌患者手术标本中TRPM6和TRPM7蛋白的表达。
在IBD和非IBD患者中，肿瘤组织中TRPM7的表达均高于相邻的非肿瘤组织。总体而言，腺癌的TRPM7表达高于腺瘤。TRPM7表达也与肿瘤分级呈正相关。相反，在IBD和非IBD结直肠癌的肿瘤组织中TRPM6表达均较高，但它与肿瘤分期或分级无关。
我们报告了TRPM6和7可能参与IBD相关和散发性结直肠癌，并表明TRPM7可能作为恶性转化和分化缺失的标志物。
