Bochniarz Mariola, Szczubiał Marek, Brodzki Piotr, Krakowski Leszek, Dąbrowski Roman
Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612, Lublin, Poland.
Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612, Lublin, Poland.
Comp Immunol Microbiol Infect Dis. 2020 Oct;72:101498. doi: 10.1016/j.cimid.2020.101498. Epub 2020 May 30.
The aim of the study was to evaluate the concentrations of amyloid A in serum (SAA) and in milk (MAA) of cows with mastitis caused by Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis and healthy cows. The blood and milk samples were obtained from Holstein-Friesian cows with clinical signs of mastitis from two tie-stall housing systems herds in the Lublin region in Poland. A total of 80 milk and serum samples from 30 cows with mastitis and 10 healthy cows were selected for study. In the quarter milk samples from cows with mastitis Streptococcus strains were isolated: Strep. agalactiae (7 cows), Strep. dysgalactiae (9 cows) and Strep. uberis (14 cows). The present study indicates that amyloid A concentration was significantly higher in milk of cows with mastitis compared to control cows (1134.25 ng/mL and 324.50 ng/mL, P < 0.001). The highest concentration of amyloid A was found in milk of cows with mastitis caused by Strep. agalactiae and Strep. uberis whereas lowest in the milk of cows with mastitis caused by Strep. dysgalactiae (3882.50 ng/mL, 2587.75 ng/mL and 812.00 ng/mL, respectively). No statistically significant difference in amyloid A concentration in serum was revealed between all unhealthy cows and control group (2140.00 ng/mL and 2510.00 ng/mL, P > 0.05). There was also no statistically significant difference between the level of amyloid A in serum and in milk of cows with mastitis caused by Strep. agalactiae and Strep. uberis. Whereas, in the case of Strep. dysgalactiae, like in the group of healthy cows, the level of amyloid A was significantly higher in serum compared to this in milk (2100 ng/mL and 812.00 ng/mL, P < 0.001; 2510.00 ng/mL and 324.50 ng/mL, P < 0.001; respectively).
本研究的目的是评估患有无乳链球菌、乳房链球菌和停乳链球菌引起的乳腺炎的奶牛以及健康奶牛血清（SAA）和乳汁（MAA）中淀粉样蛋白A的浓度。血液和乳汁样本取自波兰卢布林地区两个拴系式牛舍系统牛群中出现乳腺炎临床症状的荷斯坦-弗里生奶牛。总共从30头患乳腺炎的奶牛和10头健康奶牛中选取了80份乳汁和血清样本进行研究。在患乳腺炎奶牛的每个乳腺的乳汁样本中分离出了链球菌菌株：无乳链球菌（7头奶牛）、乳房链球菌（9头奶牛）和停乳链球菌（14头奶牛）。本研究表明，与对照奶牛相比，患乳腺炎奶牛乳汁中淀粉样蛋白A的浓度显著更高（分别为1134.25 ng/mL和324.50 ng/mL，P < 0.001）。在由无乳链球菌和停乳链球菌引起的乳腺炎奶牛的乳汁中发现淀粉样蛋白A的浓度最高，而在由乳房链球菌引起的乳腺炎奶牛的乳汁中浓度最低（分别为3882.50 ng/mL、2587.75 ng/mL和812.00 ng/mL）。在所有患病奶牛和对照组之间，血清中淀粉样蛋白A的浓度没有显示出统计学上的显著差异（分别为2140.00 ng/mL和2510.00 ng/mL，P > 0.05）。由无乳链球菌和停乳链球菌引起的乳腺炎奶牛血清和乳汁中淀粉样蛋白A的水平之间也没有统计学上的显著差异。然而，对于乳房链球菌，与健康奶牛组一样，血清中淀粉样蛋白A的水平显著高于乳汁中的水平（分别为2100 ng/mL和812.00 ng/mL，P < 0.001；2510.00 ng/mL和324.50 ng/mL，P < 0.001）。
