CIRAD, UMR PVBMT, 97410, Saint-Pierre, La Réunion, France.
Université de La Réunion, UMR PVBMT, 97410, Saint-Pierre, La Réunion, France.
Arch Virol. 2020 Aug;165(8):1925-1928. doi: 10.1007/s00705-020-04685-5. Epub 2020 Jun 6.
Nine complete nucleotide sequences of geminialphasatellites (subfamily Geminialphasatellitinae, family Alphasatellitidae) recovered from the wild Poaceae Sorghum arundinaceum collected in Reunion are described and analyzed. While the helper geminivirus was identified as an isolate of maize streak virus (genus Mastrevirus, family Geminiviridae), the geminialphasatellite genomes were most closely related to, and shared ~63% identity with, clecrusatellites. Even though the geminialphasatellite molecules lack an adenine rich-region, they have the typical size of geminialphasatellites, encode a replication-associated protein in the virion sense, and have probable stem-loop structures at their virion-strand origins of replication. According to the proposed geminialphasatellite species and genus demarcation thresholds (88% and 70% nucleotide identity, respectively), the genomes identified here represent a new species (within a new genus) for which we propose the name "Sorghum mastrevirus-associated alphasatellite" (genus "Sorgasalphasatellite").
从留尼汪野生高粱中回收的九种完整的双生阿尔法卫星（双生阿尔法卫星亚科，阿尔法卫星科）的核苷酸序列被描述和分析。虽然辅助双生病毒被鉴定为玉米条纹病毒（属马斯特里夫病毒，双生病毒科）的分离物，但双生阿尔法卫星基因组与 clecrusatellites 最为密切相关，并且共享约 63%的同一性。尽管双生阿尔法卫星分子缺乏富含腺嘌呤的区域，但它们具有双生阿尔法卫星的典型大小，在病毒粒子 sense 中编码复制相关蛋白，并且在其病毒链复制起点处具有可能的茎环结构。根据拟议的双生阿尔法卫星种和属的划分阈值（分别为 88%和 70%的核苷酸同一性），这里鉴定的基因组代表了一个新的种（在一个新的属中），我们建议将其命名为“高粱马斯特里夫病毒相关阿尔法卫星”（属“Sorgasalphasatellite”）。
