State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China.
CORTEVA™ Agriscience, Shanghai, China.
Pest Manag Sci. 2020 Dec;76(12):3975-3981. doi: 10.1002/ps.5946. Epub 2020 Jul 6.
Oxathiapiprolin, developed by DuPont, is the only commercial oxysterol-binding protein inhibitor (OSBPI) of oomycete pathogens. Although the activity of oxathiapiprolin on some Pythium spp. and Phytopythium spp. has been reported, it has not been tested on many other species, and little is known about the mechanisms of Pythium spp. that are tolerant to it.
Oxathiapiprolin exhibited a strong inhibitory effect on mycelial growth of Phy. litorale, Phy. helicoides and Phy. chamaehyphon, with EC values ranging from 0.002 to 0.013 μg mL . It also showed good effectiveness against Py. splendens and two Py. ultimum isolates, with EC values ranging from 0.167 to 0.706 μg mL , but showed no activity against 14 other Pythium spp. Oxathiapiprolin provoked a slight upregulation of PuORP1 in Py. ultimum, but it did not lead to PaORP1-1 or PaORP1-2 overexpression in Py. aphanidermatum. Transformation and expression of PuORP1, PaORP1-1 or PaORP1-2 in the sensitive wild-type Phytophthora sojae isolate P6497 confirmed that either the PuORP1, PaORP1-1 or PaORP1-2 was responsible for the observed oxathiapiprolin tolerance.
This study showed that oxathiapiprolin had excellent activity against Phytopythium spp. but displayed a differentiated activity against different Pythium spp. ORP1s in Pythium spp. are positively related to the tolerance of Pythium species to oxathiapiprolin. © 2020 Society of Chemical Industry.
由杜邦公司开发的唑吡普林是唯一的商业氧化固醇结合蛋白抑制剂（OSBPI）杀卵菌病原体。虽然唑吡普林对一些腐霉属和疫霉属物种的活性已经有报道，但它尚未在许多其他物种上进行测试，并且对耐受它的腐霉属物种的机制知之甚少。
唑吡普林对Phy.litorale、Phy.helicoides 和 Phy.chamaehyphon 的菌丝生长表现出很强的抑制作用，EC 值范围为 0.002 至 0.013μg·mL-1。它对Py.splendens 和两个Py.ultimum 分离株也表现出良好的效果，EC 值范围为 0.167 至 0.706μg·mL-1，但对其他 14 种腐霉属物种没有活性。唑吡普林轻微上调了Py.ultimum 中的 PuORP1，但在Py.aphanidermatum 中没有导致 PaORP1-1 或 PaORP1-2 过表达。敏感野生型大豆疫霉分离株 P6497 中 PuORP1、PaORP1-1 或 PaORP1-2 的转化和表达证实，要么 PuORP1，要么 PaORP1-1 要么 PaORP1-2 负责观察到的唑吡普林耐受性。
本研究表明唑吡普林对Phytopythium spp.具有极好的活性，但对不同的腐霉属物种表现出不同的活性。腐霉属中的 ORP1 与腐霉属物种对唑吡普林的耐受性呈正相关。© 2020 化学工业协会。
