Thermo Fisher Scientific, Uppsala, Sweden.
Laboratoire Synlab Provence, Marseille, France.
Clin Exp Allergy. 2020 Aug;50(8):964-972. doi: 10.1111/cea.13675. Epub 2020 Jun 25.
Severe allergy to fruits mediated by a 7 kDa allergen belonging to the gibberellin-regulated protein (GRP) family is known to be associated with Cupressaceae pollinosis.
To identify and characterize Cupressaceae pollen allergens involved in GRP-related fruit allergy.
Pru p 7-related proteins from pollen of Cupressus sempervirens, Juniperus ashei and Cryptomeria japonica were identified using a rabbit anti-Pru p 7 antiserum, purified chromatographically and sequenced by mass spectrometry and bioinformatic comparisons. The C sempervirens protein was produced as a recombinant allergen in Pichia pastoris. IgE antibody binding to pollen GRP proteins was analysed in a peach allergic (n = 54) and a cypress pollen allergic (n = 88) patient population from southern France using ImmunoCAP.
In each of the three Cupressaceae species studied, a 7 kDa pollen protein related to Pru p 7 was identified and found to comprise an amino acid sequence of 63 residues in length, 92%-98% identical to each other and 67%-68% identical to Pru p 7. The C sempervirens, J ashei and C japonica GRP allergens have been officially recognized by the WHO/IUIS Allergen Nomenclature Sub-Committee and named Cup s 7, Jun a 7 and Cry j 7, respectively. Recombinant Cup s 7 showed IgE antibody binding capacity comparable to that of the purified natural allergen. Among 51 peach allergic subjects sensitized to Pru p 7, substantially higher levels of IgE to Cup s 7 than to Pru p 7 were found. Further, the pollen protein was able to completely outcompete IgE binding to Pru p 7, while the reverse competition effect was modest, consistent with primary sensitization by the pollen allergen.
Pru p 7-related pollen allergens from three Cupressaceae species have been characterized and may become useful for the identification of pollinosis patients at risk of developing severe fruit allergy.
已知 7 kDa 过敏原属于赤霉素调节蛋白（GRP）家族，介导的水果过敏与柏科花粉过敏有关。
鉴定和描述与 GRP 相关的果实过敏有关的柏科花粉过敏原。
使用兔抗普鲁普 7 抗血清鉴定来自柏木、刺柏和日本柳杉花粉的普鲁普 7 相关蛋白，通过质谱和生物信息学比较进行纯化和测序。将 C sempervirens 蛋白作为重组过敏原在毕赤酵母中生产。使用 ImmunoCAP 分析来自法国南部的 54 名桃过敏患者和 88 名柏科花粉过敏患者的花粉 GRP 蛋白的 IgE 抗体结合。
在所研究的三种柏科植物中，鉴定出与普鲁普 7 相关的 7 kDa 花粉蛋白，其氨基酸序列长度为 63 个残基，彼此之间 92%-98%相同，与普鲁普 7 相同 67%-68%。C sempervirens、J ashei 和 C japonica GRP 过敏原已被世界卫生组织/国际免疫学会联合会过敏原命名小组委员会正式认可，并分别命名为 Cup s 7、Jun a 7 和 Cry j 7。重组 Cup s 7 显示出与纯化天然过敏原相当的 IgE 抗体结合能力。在 51 名对普鲁普 7 过敏的桃过敏患者中，发现对 Cup s 7 的 IgE 水平明显高于对普鲁普 7 的水平。此外，花粉蛋白能够完全竞争 IgE 与普鲁普 7 的结合，而反向竞争效应适度，与花粉过敏原的初次致敏一致。
从三种柏科植物中鉴定出与普鲁普 7 相关的花粉过敏原，可能成为鉴定有发生严重水果过敏风险的花粉过敏患者的有用工具。
