美国非 SLE 女护士中每日饮酒与系统性红斑狼疮相关细胞因子和趋化因子的关系。
Associations between daily alcohol consumption and systemic lupus erythematosus-related cytokines and chemokines among US female nurses without SLE.
机构信息
Division of Rheumatology, Inflammation and Immunity, Brigham and Women's Hospital, Boston, USA.
Channing Division of Network Medicine, Brigham and Women's Hospital, Boston, USA.
出版信息
Lupus. 2020 Jul;29(8):976-982. doi: 10.1177/0961203320929427. Epub 2020 Jun 6.
OBJECTIVES
Moderate alcohol consumption has been associated with decreased systemic lupus erythematosus risk, but the biologic basis for this association is unknown. We aimed to determine whether moderate alcohol consumption was associated with lower concentrations of systemic lupus erythematosus-associated chemokines/cytokines in an ongoing cohort of female nurses without systemic lupus erythematosus, and whether the association was modified by the presence of systemic lupus erythematosus-related autoantibodies.
METHODS
About 25% of participants from the Nurses' Health Study ( = 121,700 women) and Nurses' Health Study 2 ( = 116,429) donated a blood sample; of these, 1177 women were without systemic lupus erythematosus at time of donation. Cumulative average and current (within 4 years) intakes of beer, wine or liquor were assessed from pre-blood draw questionnaires. Chemokine/cytokine concentrations (stem cell factor, B-lymphocyte stimulator, interferon-inducible protein-10, interferon-alpha, interleukin-10) and antibodies against dsDNA and extractable nuclear antigens were obtained using enzyme-linked immunosorbent assays. Antinuclear antibodies were detected by indirect immunofluorescence on HEp-2 cells.
RESULTS
At blood draw, the women's mean age was 56 years and 22% were antinuclear antibody positive; 36% were African-American. About half (46%) reported consuming 0-5 g/day of alcohol. Stem cell factor levels were 0.5% lower ( < 0.0001) for every gram per day increase in cumulative average alcohol consumption. Women who consumed >5 g/day had mean stem cell factor levels 7% lower ( = 0.002) than non-drinkers. Other cytokines were not significantly associated with alcohol intake. Autoantibody status did not modify observed associations.
CONCLUSION
In this study of female nurses, moderate alcohol consumption was associated with lower stem cell factor levels, suggesting a plausible mechanism through which alcohol may lower systemic lupus erythematosus risk might be by decreasing circulating stem cell factor.
目的
适量饮酒与系统性红斑狼疮风险降低有关，但这种关联的生物学基础尚不清楚。我们旨在确定在一个正在进行的、没有系统性红斑狼疮的女性护士队列中，适量饮酒是否与系统性红斑狼疮相关趋化因子/细胞因子的浓度降低有关，以及这种关联是否因存在系统性红斑狼疮相关自身抗体而改变。
方法
来自护士健康研究（ = 121700 名女性）和护士健康研究 2 期（ = 116429 名）的约 25%的参与者捐献了血液样本；其中，1177 名女性在捐献时没有系统性红斑狼疮。通过预采血前的问卷评估啤酒、葡萄酒或白酒的累积平均和当前（4 年内）摄入量。使用酶联免疫吸附试验测定趋化因子/细胞因子浓度（干细胞因子、B 淋巴细胞刺激因子、干扰素诱导蛋白-10、干扰素-α、白细胞介素-10）和针对 dsDNA 和可提取核抗原的抗体。通过在 Hep-2 细胞上进行间接免疫荧光检测抗核抗体。
结果
在采血时，女性的平均年龄为 56 岁，22%的女性抗核抗体阳性；36%为非裔美国人。约一半（46%）报告每天摄入 0-5 克酒精。累积平均酒精摄入量每增加 1 克/天，干细胞因子水平就会降低 0.5%（ < 0.0001）。每天摄入>5 克酒精的女性，其平均干细胞因子水平比不饮酒者低 7%（ = 0.002）。其他细胞因子与酒精摄入量无显著相关性。自身抗体状态并未改变观察到的关联。
结论
在这项对女性护士的研究中，适量饮酒与较低的干细胞因子水平相关，这表明酒精可能通过降低循环干细胞因子水平来降低系统性红斑狼疮风险的一种可能机制。
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