Kamstrup Sabine Lerke, Schmidt Nanna Surlemont, Langkilde Henrik Zachar, Nilsson Anna Christine, Voss Anne
Institute of Regional Health Research, University of Southern Denmark, Odense, Denmark
Department of Rheumatology, Odense University Hospital, Odense, Denmark.
Lupus Sci Med. 2025 Aug 17;12(2):e001550. doi: 10.1136/lupus-2025-001550.
The primary objective was to assess the prevalence of anti-ribosomal-P protein antibodies (anti-Rib-P) in a well-characterised Danish adult SLE cohort. Secondary objectives included (1) assessing any association between anti-Rib-P and neuropsychiatric SLE (NPSLE), (2) assessing any association between anti-Rib-P and characteristics of Danish patients with SLE and (3) assessing potential associations between selected autoantibodies and NPSLE.
This cross-sectional study included 198 Danish patients with SLE from a population-based cohort. Patients meet the American College of Rheumatology's 1997 revised classification criteria for SLE and were 18 years of age or older. Anti-Rib-P were detected in serum using fluorescence enzyme immunoassay. ANA patterns were tested using indirect immunofluorescence on HEp-2 cells. Anti-double-stranded DNA antibody was examined by manual ELISA, and antibodies targeting Ro/SSA, La/SSB, Sm, RNP, Scl-70, Jo-1, Centromer B and Histone were examined on a Luminex instrument.
We identified 14 (7.1%) patients with anti-Rib-P positive SLE, and 3 anti-Rib-P positive patients were diagnosed with NPSLE (21% of patients with NPSLE). There was no statistically significant association between anti-Rib-P and NPSLE. The mean anti-Rib-P titres in the patients with NPSLE did not differ significantly from patients without NPSLE patients' titres. We observed a significant association between anti-Rib-P positivity and disease activity measured by the SLEDAI-2K (SLE Disease Activity Index 2000) score. A stepwise logistic regression found an association between NPSLE and SLEDAI-2K and SLICC DI (SLE International Collaborating Clinics Damage Index) score.
This study could not confirm a role for anti-Rib-P in the identification of NPSLE. On the other hand, anti-Rib-P was associated with SLE disease activity as well as organ damage.
主要目的是评估在一个特征明确的丹麦成年系统性红斑狼疮（SLE）队列中抗核糖体P蛋白抗体（抗Rib-P）的患病率。次要目的包括：（1）评估抗Rib-P与神经精神性SLE（NPSLE）之间的任何关联；（2）评估抗Rib-P与丹麦SLE患者特征之间的任何关联；（3）评估选定自身抗体与NPSLE之间的潜在关联。
这项横断面研究纳入了来自一个基于人群队列的198例丹麦SLE患者。患者符合美国风湿病学会1997年修订的SLE分类标准，年龄在18岁及以上。采用荧光酶免疫分析法检测血清中的抗Rib-P。使用间接免疫荧光法在HEp-2细胞上检测抗核抗体（ANA）模式。通过手工酶联免疫吸附测定法检测抗双链DNA抗体，并在Luminex仪器上检测针对Ro/SSA、La/SSB、Sm、RNP、Scl-70、Jo-1、着丝粒B和组蛋白的抗体。
我们鉴定出14例（7.1%）抗Rib-P阳性的SLE患者，3例抗Rib-P阳性患者被诊断为NPSLE（占NPSLE患者的21%）。抗Rib-P与NPSLE之间无统计学显著关联。NPSLE患者的抗Rib-P平均滴度与无NPSLE患者的滴度无显著差异。我们观察到抗Rib-P阳性与采用SLEDAI-2K（2000年SLE疾病活动指数）评分所衡量的疾病活动之间存在显著关联。逐步逻辑回归发现NPSLE与SLEDAI-2K及SLICC DI（SLE国际协作临床损伤指数）评分之间存在关联。
本研究无法证实抗Rib-P在NPSLE识别中的作用。另一方面，抗Rib-P与SLE疾病活动以及器官损伤相关。
