Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China.
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China.
Biochem Biophys Res Commun. 2020 Jul 30;528(3):538-544. doi: 10.1016/j.bbrc.2020.05.153. Epub 2020 Jun 4.
Glutathione S-transferases (GSTs) play important roles in metabolism and detoxification of plant cells. However, their functions during development are less well understood. Arabidopsis AtGSTU7 (AT2G29420) encodes a Tau class GST. Here we provide the AtGSTU7 was abundantly expressed in seeds and in roots at an early stage of germination. AtGSTU7 expression was repressed by exogenous ABA and promoted by osmotic stress. A null mutant of AtGSTU7 (atgstu7) accumulated higher contents of reduced GSH and decreased amounts of endogenous HO in seedlings. The atgstu7 plants showed decreased osmotic tolerance during seed germination, which was influenced by GSH and ABI3 gene expression. The results suggested that AtGSTU7 involvement in seed germination is mediated by GSH-ROS homeostasis and ABA signaling.
谷胱甘肽 S-转移酶（GSTs）在植物细胞的代谢和解毒中发挥重要作用。然而，它们在发育过程中的功能还不太清楚。拟南芥 AtGSTU7（AT2G29420）编码一个 Tau 类 GST。在这里，我们发现 AtGSTU7 在种子中大量表达，并在萌发的早期在根中表达。AtGSTU7 的表达受到外源 ABA 的抑制，受到渗透胁迫的促进。AtGSTU7 的一个缺失突变体（atgstu7）在幼苗中积累了更高水平的还原型 GSH 和更低水平的内源 HO。atgstu7 植物在种子萌发期间表现出较低的渗透耐受性，这受到 GSH 和 ABI3 基因表达的影响。结果表明，AtGSTU7 参与种子萌发是通过 GSH-ROS 平衡和 ABA 信号转导来介导的。
