Division of Microbiology, National Institute of Health Sciences.
Department of Life and Environmental Sciences, Azabu University.
Biocontrol Sci. 2020;25(2):113-118. doi: 10.4265/bio.25.113.
Aspergillus section Versicolores species, except Aspergillus sydowii, produce a carcinogenic mycotoxin sterigmatocystin (STC). Since these fungi are found in varied environmental milieu including indoor dust and food products, our aim was to develop a sensitive and convenient assay to detect STC producing fungal strains. We made use of a high discrimination DNA polymerase (HiDi DNA polymerase), for single nucleotide polymorphism (SNP)-based PCR amplification. Using specific primer pairs based on the SNPs between A. sydowii and other strains of Aspergillus section Versicolores, we succeeded in amplifying the genomic DNA all target strains except A. sydowii. These results confirm that the SNP-based PCR amplification technique, using a high discrimination DNA polymerase, was a reliable and robust screening method for target fungal strains.
除黄曲霉（Aspergillus sydowii）外，青霉属（Aspergillus section Versicolores）的一些种也会产生一种致癌的真菌毒素——桔青霉素（STC）。由于这些真菌存在于各种环境中，包括室内灰尘和食品，我们的目的是开发一种灵敏且方便的检测方法来检测产生桔青霉素的真菌菌株。我们利用高分辨力 DNA 聚合酶（HiDi DNA polymerase）进行基于单核苷酸多态性（SNP）的 PCR 扩增。使用基于 A. sydowii 和青霉属（Aspergillus section Versicolores）其他种之间 SNP 的特异性引物对，我们成功地扩增了除 A. sydowii 之外的所有目标菌株的基因组 DNA。这些结果证实，使用高分辨力 DNA 聚合酶的 SNP 基 PCR 扩增技术是一种可靠且强大的目标真菌菌株筛选方法。
