Faculty of Dentistry, Department of Restorative Dentistry, Ankara University.
Biotechnology Institution, Sisbiyotek, Ankara University.
Dent Mater J. 2020 Sep 29;39(5):815-824. doi: 10.4012/dmj.2019-221. Epub 2020 Jun 6.
The aim of this study was to evaluate the cytotoxicity of universal adhesives on L929 mouse fibroblast cell line by using a real-time cell analysis. In order to obtain extract, six different cured dental adhesives were immersed in Dulbecco's Modified Eagle's Medium (DMEM) at 37°C for 24 h. A real-time cell analysis system was used to assess cytotoxicity of the dental adhesives. After seeding 25,000 cells/300 μL/well cell suspensions into the wells of an e-plate, fibroblasts were exposed to extracts of tested adhesives at varying dilutions (1:1, 1:2, and 1:10) and observed at every 30 min intervals for 72 h. Three-way ANOVA one factor repeated measures were used to analyze the results (α=0.05). All tested adhesives induced cell viability loss, cell morphology alteration, and cell death depending on extract concentration and time. Cell viability of L929 cells to between 44 and 10% for 1:1 diluted extracts, at 72 h, when compared to the negative control.
本研究旨在通过实时细胞分析评估通用型粘结剂对 L929 小鼠成纤维细胞系的细胞毒性。为了获得浸提液，将六种不同固化的牙科粘结剂在 37°C 的 Dulbecco 改良 Eagle 培养基（DMEM）中浸泡 24 小时。使用实时细胞分析系统评估牙科粘结剂的细胞毒性。将 25000 个细胞/300μL/孔细胞悬液接种到 e-plate 的孔中后，将成纤维细胞暴露于不同稀释度（1:1、1:2 和 1:10）的测试粘结剂的浸提液中，并在 72 小时内每隔 30 分钟观察一次。采用三因素方差分析（one-factor repeated measures）对结果进行分析（α=0.05）。所有测试的粘结剂都会根据浸提液浓度和时间诱导细胞活力丧失、细胞形态改变和细胞死亡。与阴性对照组相比，1:1 稀释的浸提液在 72 小时时，L929 细胞的细胞活力下降至 44%至 10%之间。
