Laboratório de Farmacologia e Biocompatibilidade Celular, Faculdade de Medicina Dentária, Universidade do Porto, Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal.
Clin Oral Investig. 2011 Dec;15(6):885-93. doi: 10.1007/s00784-010-0464-0. Epub 2010 Sep 16.
Ten commercially available denture adhesives, nine soluble formulations (six creams, three powders) and one insoluble product (pad), were analyzed regarding the cytotoxicity profile in direct and indirect assays using L929 fibroblast cells. In the direct assay, fibroblasts were seeded over the surface of a thick adhesive gel (5%, creams; 2.5%, powders and pad). In the indirect assay, cells were cultured in the presence of adhesive extracts prepared in static and dynamic conditions (0.5-2%, creams; 0.25-1%, powders and pad). Cell toxicity was assessed for cell viability/proliferation (MTT assay) and cell morphology (observation of the F-actin cytoskeleton organization by confocal laser scanning microscopy). Direct contact of the L929 fibroblasts with the thick adhesive gels caused no, or only a slight, decrease in cell viability/proliferation. The adhesive extracts (especially those prepared in dynamic conditions) caused significantly higher growth inhibition of fibroblasts and, in addition, caused dose- and time-dependent effects, throughout the 6-72 h exposure time. Also, dose-dependent effects on cell morphology, with evident disruption of the F-actin cytoskeleton organization, were seen in the presence of most adhesives. In conclusion, the adhesives possessed different degrees of cytotoxicity, but similar dose- and time-dependent biological profiles.
对 10 种市售义齿黏附剂（9 种可溶性制剂，包括 6 种乳膏、3 种粉剂；1 种不溶性制剂，即垫）进行了分析，采用 L929 成纤维细胞，直接和间接检测法检测细胞毒性特征。在直接检测法中，将成纤维细胞播种于厚黏附凝胶（乳膏为 5%，粉剂和垫为 2.5%）表面。在间接检测法中，将细胞在静态和动态条件下制备的黏附提取物（乳膏为 0.5-2%，粉剂和垫为 0.25-1%）存在的情况下培养。通过 MTT 测定法评估细胞活力/增殖（MTT 测定法）和细胞形态（通过共焦激光扫描显微镜观察 F-肌动蛋白细胞骨架组织）评估细胞毒性。L929 成纤维细胞与厚黏附凝胶直接接触不会导致细胞活力/增殖降低，或仅有轻微降低。黏附提取物（特别是在动态条件下制备的提取物）会导致成纤维细胞生长明显受到抑制，此外，在 6-72 小时暴露时间内，还会产生剂量和时间依赖性效应。在大多数黏附剂存在的情况下，还观察到对细胞形态的剂量依赖性影响，F-肌动蛋白细胞骨架组织明显受到破坏。总之，黏附剂具有不同程度的细胞毒性，但具有相似的剂量和时间依赖性生物学特征。
