miR-140-5p的下调通过靶向早期生长反应蛋白2（EGR2）影响先天性巨结肠（HSCR）的发病机制。
Downregulation of miR-140-5p affects the pathogenesis of HSCR by targeting EGR2.
作者信息
Du Guoqiang, Wang Xiaoqing, Wu Yidi, Zhang Yongfei, Liu Wei, Wu Rongde
机构信息
Department of Pediatric Surgery, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Street, Jinan, 250021, Shandong, People's Republic of China.
Department of Dermatology, The First Affiliated Hospital of Shandong First Medical University, Jinan, People's Republic of China.
出版信息
Pediatr Surg Int. 2020 Aug;36(8):883-890. doi: 10.1007/s00383-020-04686-0. Epub 2020 Jun 7.
BACKGROUND/AIMS: Hirschsprung's disease (HSCR) is the most common digestive disease caused by disorders of neural crest development. Despite the known involvement of miR-140-5p in many human diseases, its biological role in Hirschsprung's disease (HSCR) remains undefined. In this study, we sought to reveal the roles of miR-140-5p in the pathogenesis of HSCR.
METHODS
Quantitative real-time PCR and western blotting were used to measure the relative expression levels of miRNAs, mRNAs, and proteins in stenotic and dilated sections of the colon of 32 HSCR patients. Targets and proteins were evaluated by western blotting, and Transwell, CCK-8, and flow cytometry assays were adopted to detect the functional effects of miR-140-5p on SH-SY5Y cells.
RESULTS
miR-140-5p was significantly downregulated in HSCR tissue samples with increased expression of EGR2, and knockdown of miR-140-5p inhibited cell migration and proliferation and promoted apoptosis in SH-SY5Y cell lines. EGR2 expression was inversely correlated with that of miR-140-5p in cell lines.
CONCLUSIONS
miR-140-5p may influence the pathogenesis of HSCR by targeting EGR2.
背景/目的：先天性巨结肠（HSCR）是神经嵴发育紊乱引起的最常见的消化系统疾病。尽管已知miR-140-5p参与多种人类疾病，但它在先天性巨结肠（HSCR）中的生物学作用仍不明确。在本研究中，我们试图揭示miR-140-5p在HSCR发病机制中的作用。
方法
采用定量实时PCR和蛋白质印迹法检测32例HSCR患者结肠狭窄和扩张段中miRNA、mRNA和蛋白质的相对表达水平。通过蛋白质印迹法评估靶标和蛋白质，并采用Transwell、CCK-8和流式细胞术检测miR-140-5p对SH-SY5Y细胞的功能影响。
结果
在HSCR组织样本中，miR-140-5p显著下调，EGR2表达增加，敲低miR-140-5p可抑制SH-SY5Y细胞系的细胞迁移和增殖，并促进其凋亡。在细胞系中，EGR2的表达与miR-140-5p的表达呈负相关。
结论
miR-140-5p可能通过靶向EGR2影响HSCR的发病机制。
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