State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Anal Chem. 2020 Jul 7;92(13):8675-8679. doi: 10.1021/acs.analchem.0c00458. Epub 2020 Jun 23.
A selective extraction method with remarkable advantages (i.e., low cost, simplicity, and solvent savings) was developed for the detection of long-chain per- and polyfluoroalkyl substances (PFASs) based on their specific protein affinities. Bovine serum albumin (BSA) was selected to extract PFASs from aqueous samples, which were then desorbed using methanol. Finally, the PFASs were analyzed by high-performance liquid chromatography-tandem mass spectrometry. The optimal extraction conditions were as follows: sample volume, 10 mL; BSA concentration, 2 g/L; equilibration time, 5 min; pH, 3.4; salinity, 6% (w/v) NaCl; and water bath temperature, 80 °C. The protein affinity property was confirmed to be the major extraction mechanism, which significantly increased the selectivity for PFASs. The recoveries of this method for 15 legacy PFASs with CF ≥ 6 and three chlorinated polyfluoroether sulfonic acids in tap water, river water, and urine samples with three spiked levels were 73.3-122.3, 83.8-119.4, and 75.0-115.1%, respectively. The method limits of quantification in the three real matrix samples were 4.7-133.3 ng/L. Comparative experiments with conventional solid-phase extraction confirm that the developed approach can be a promising and alternative method for the extraction of PFASs from authentic aqueous samples. Moreover, the proposed method provides a new possibility for screening PFASs exhibiting high bioaccumulation and toxicity.
一种具有显著优势（即低成本、简单和节省溶剂）的选择性萃取方法被开发用于基于其与蛋白质的特异性亲和力来检测长链全氟和多氟烷基物质（PFASs）。牛血清白蛋白（BSA）被选择用于从水样中萃取 PFASs，然后用甲醇解吸。最后，通过高效液相色谱-串联质谱法对 PFASs 进行分析。最佳萃取条件如下：样品体积，10 mL；BSA 浓度，2 g/L；平衡时间，5 min；pH 值，3.4；盐度，6%（w/v）NaCl；水浴温度，80°C。蛋白质亲和力特性被确认为主要的萃取机制，这显著提高了对 PFASs 的选择性。该方法对自来水中的 15 种具有 CF≥6 的传统 PFASs 和三种氯化聚氟醚磺酸，以及三种加标水平的尿液样品中的回收率为 73.3-122.3、83.8-119.4 和 75.0-115.1%。在三种实际基质样品中的方法检出限为 4.7-133.3 ng/L。与传统固相萃取的对比实验证实，该方法可为从实际水样中萃取 PFASs 提供一种有前途的替代方法。此外，该方法为筛选具有高生物累积性和毒性的 PFASs 提供了一种新的可能性。
