Siddiqui Safia, Jaiswal Rohit, Hashmi Ghulam Sarwar
Department of Oral Pathology and Microbiology, Sardar Patel Post Graduate Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India.
Department of Oral and Maxillofacial Surgery, ZA Dental College and Hospital, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
J Oral Maxillofac Pathol. 2020 Jan-Apr;24(1):131-137. doi: 10.4103/jomfp.JOMFP_70_18. Epub 2020 May 8.
Stromal response to cancer is usually characterized by intense lymphoplasmacytic infiltrate. However, recently, the attention has shifted to tumor-associated tissue eosinophils (TATE). Tumor-associated blood eosinophils (TABE) are rare in solid cancers; however, carcinoma of the head and neck shows its prevalence.
The aim of the study was to investigate the prevalence and relationship of tissue and blood eosinophils in various grades of oral cancer. The purpose of the article is to emphasize the possible clinical and biological significance of eosinophils in patients of oral squamous cell carcinoma (OSCC) so that appropriate therapeutic strategies can be devised accordingly.
Thirty histologically confirmed cases of oral squamous cell carcinoma were divided into well, moderate and poorly differentiated carcinoma. Eosinophilic infiltration in the tissue was graded as low, moderate and massive TATE. The number of eosinophils per 100 WBCs was taken as the differential eosinophil count. Blood eosinophilia (BE) >6% was considered to be TABE.
Hematoxylin and eosin-stained tissue sections at 5 μ were evaluated. Prolonged staining in dilute 0.05% aqueous eosin demonstrated eosinophils selectively. Blood smears were stained by Leishman stain.
Student's -test, Chi-square test, ANOVA, Newman-Keuls Multiple Comparison Test and Karl Pearson correlation coefficient® method were used.
The mean TATE value was highest in poorly differentiated carcinoma. TABE was seen only in a few cases and was associated mostly with poorly differentiated OSCC.
There was a statistically significant correlation between TATE and histological grades of OSCC. Eosinophilia of the peripheral blood is an adverse sign in patients with carcinoma.
癌症的基质反应通常以强烈的淋巴浆细胞浸润为特征。然而，最近，注意力已转向肿瘤相关组织嗜酸性粒细胞（TATE）。肿瘤相关血液嗜酸性粒细胞（TABE）在实体癌中很少见；然而，头颈癌显示出其普遍性。
本研究的目的是调查不同分级口腔癌中组织和血液嗜酸性粒细胞的患病率及关系。本文的目的是强调嗜酸性粒细胞在口腔鳞状细胞癌（OSCC）患者中可能的临床和生物学意义，以便据此制定适当的治疗策略。
30例经组织学确诊的口腔鳞状细胞癌病例分为高分化、中分化和低分化癌。组织中的嗜酸性粒细胞浸润分为低、中、大量TATE。每100个白细胞中的嗜酸性粒细胞数量作为嗜酸性粒细胞分类计数。血液嗜酸性粒细胞增多（BE）>6%被认为是TABE。
评估5μm苏木精和伊红染色的组织切片。在稀释的0.05%水性伊红中长时间染色可选择性地显示嗜酸性粒细胞。血液涂片用瑞氏染色。
采用学生t检验、卡方检验、方差分析、纽曼-基尔斯多重比较检验和卡尔·皮尔逊相关系数法。
低分化癌的平均TATE值最高。仅在少数病例中发现TABE，且大多与低分化OSCC相关。
TATE与OSCC的组织学分级之间存在统计学显著相关性。外周血嗜酸性粒细胞增多是癌症患者的不良征象。
