Joshi Priya Shirish, Kaijkar Manasi S
Department of Oral Pathology and Microbiology, Vasantdada Patil Dental College and Hospital, Kavalapur, Sangli, Maharashtra, India.
Dent Res J (Isfahan). 2013 Nov;10(6):784-9.
Tumor associated tissue eosinophilia is believed to play a significant role in the biological behavior of the carcinoma. Eosinophil infiltrate in association with the head and neck squamous cell carcinoma (SCC) have been reviewed from time-to-time. The significance of such an association has been variably thought to be either a potential diagnostic tool for stromal invasion or as a prognostic indicator. We aimed to evaluate the efficacy of Congo red staining to differentiate eosinophils in the inflammatory infiltrate in oral squamous cell carcinoma (OSCC) and whether this eosinophilia is associated with the histologic grading in OSCC.
The eosinophil infiltration in hematoxylin and eosin (H and E) and Congo red stained sections of 50 biopsies of OSCC were examined. The eosinophil distribution was quantitatively evaluated in both sections as either diffuse or focal and scored as mild, moderate and severe grades. The average number of eosinophils obtained in OSCC stained by H and E and Congo red were then statistically compared by univariate analysis carried out using Student's t-test. P < 0.01 was used to determine the statistical significance.
In the OSCC, the eosinophil infiltration was seen in 43 out of 50 (86%) cases. The elevation of eosinophil count was more diffuse than focal (36 vs. 7 [83.72 vs. 16.27%]). The staining efficacy of Congo red stain over H and E stain to differentiate eosinophils was excellent and found to be statistically significant (P < 0.01). No significant correlation was found with the eosinophil infiltration and the histologic grades of OSCC.
Our study showed a strong infiltration of eosinophils in OSCC though no significant correlation was found with the eosinophil infiltration and the histologic grades of OSCC. Congo red staining showed a high sensitivity in staining eosinophils over routine H and E. This staining technique could therefore provide an adjunct to routine H and E in evaluating eosinophils in dysplasia and OSCC cases.
肿瘤相关组织嗜酸性粒细胞增多被认为在癌的生物学行为中起重要作用。头颈部鳞状细胞癌（SCC）相关的嗜酸性粒细胞浸润已被不时回顾。这种关联的意义一直被不同地认为要么是间质浸润的潜在诊断工具，要么是预后指标。我们旨在评估刚果红染色在鉴别口腔鳞状细胞癌（OSCC）炎症浸润中的嗜酸性粒细胞方面的效果，以及这种嗜酸性粒细胞增多是否与OSCC的组织学分级相关。
检查了50例OSCC活检标本苏木精和伊红（H&E）染色及刚果红染色切片中的嗜酸性粒细胞浸润情况。在两个切片中对嗜酸性粒细胞分布进行定量评估，分为弥漫性或局灶性，并分为轻度、中度和重度等级。然后通过使用学生t检验进行单因素分析，对H&E染色和刚果红染色的OSCC中获得的嗜酸性粒细胞平均数量进行统计学比较。P<0.01用于确定统计学意义。
在OSCC中，50例中有43例（86%）可见嗜酸性粒细胞浸润。嗜酸性粒细胞计数升高以弥漫性为主，而非局灶性（36例对7例[83.72%对16.27%]）。刚果红染色在鉴别嗜酸性粒细胞方面优于H&E染色，且具有统计学意义（P<0.01）。未发现嗜酸性粒细胞浸润与OSCC的组织学分级之间存在显著相关性。
我们的研究表明OSCC中有强烈的嗜酸性粒细胞浸润，尽管未发现嗜酸性粒细胞浸润与OSCC的组织学分级之间存在显著相关性。刚果红染色在染色嗜酸性粒细胞方面比常规H&E具有更高的敏感性。因此，这种染色技术可在评估发育异常和OSCC病例中的嗜酸性粒细胞时作为常规H&E的辅助手段。
