不同组织学分级口腔鳞状细胞癌中肿瘤相关组织嗜酸性粒细胞增多症的定量分析
Quantitative analysis of tumor-associated tissue eosinophilia in different histological grades of oral squamous cell carcinoma.
作者信息
Bankur Rashmi, Rodrigues Charlotte, Anjaly D, Gopinathan Pillai Arun, Bankur Praveen Kumar
机构信息
Department of Service Clinic, Government Dental College and Research Institute, Bengaluru, Karnataka, India.
Department of Oral and Maxillofacial Pathology, MR Ambedkar Dental College, Bengaluru, Karnataka, India.
出版信息
Indian J Dent Res. 2016 Sep-Oct;27(5):463-467. doi: 10.4103/0970-9290.195612.
BACKGROUND AND OBJECTIVES
Oral squamous cell carcinoma (OSCC) is the 8 th most common aggressive epithelial malignant neoplasm worldwide today. The eosinophil may be a "multifaceted cell" that can be associated with wound-healing processes, as well as to tissue damage which has increased the speculations around tumor-associated tissue eosinophilia in malignant tumors. The aim of this study was to detect the role and quantitative analysis of tumor-associated tissue eosinophils in different histological grades of OSCC.
MATERIALS AND METHODS
A retrospective study was carried out in sixty cases of histopathologically graded OSCCs. Tissue sections of 4 μ thickness were made from paraffin-embedded tissue blocks and were stained with hematoxylin and eosin. Eosinophils were counted under randomly selected twenty high-power (×40) fields. Data were subjected to statistical analysis using ANOVA test.
RESULTS
Higher mean eosinophils were recorded in well-differentiated squamous cell carcinoma (WDSCC) followed by moderately differentiated squamous cell carcinoma (MDSCC) and poorly differentiated squamous cell carcinoma (PDSCC) groups, respectively. The difference in mean eosinophils was found to be statistically significant between WDSCC and MDSCC (P < 0.001), as well as between WDSCC and PDSCC (P < 0.001).
CONCLUSION
Tumor-associated tissue eosinophil count is higher in WDSCC as compared to moderate and PDSCC.
背景与目的
口腔鳞状细胞癌（OSCC）是当今全球第八大常见的侵袭性上皮恶性肿瘤。嗜酸性粒细胞可能是一种“多面细胞”，它既与伤口愈合过程相关，也与组织损伤有关，这增加了人们对恶性肿瘤中肿瘤相关组织嗜酸性粒细胞增多现象的猜测。本研究的目的是检测肿瘤相关组织嗜酸性粒细胞在不同组织学分级的OSCC中的作用并进行定量分析。
材料与方法
对60例经组织病理学分级的OSCC病例进行回顾性研究。从石蜡包埋的组织块制作4μm厚的组织切片，并用苏木精和伊红染色。在随机选择的20个高倍（×40）视野下对嗜酸性粒细胞进行计数。数据采用方差分析进行统计分析。
结果
在高分化鳞状细胞癌（WDSCC）组中记录到的平均嗜酸性粒细胞数量较高，其次分别是中分化鳞状细胞癌（MDSCC）组和低分化鳞状细胞癌（PDSCC）组。发现WDSCC与MDSCC之间（P < 0.001）以及WDSCC与PDSCC之间（P < 0.001）平均嗜酸性粒细胞数量的差异具有统计学意义。
结论
与中分化和低分化鳞状细胞癌相比，高分化鳞状细胞癌中肿瘤相关组织嗜酸性粒细胞计数更高。
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