Ding Wensen, Wang Yuqin, Cheng Yaqin, Chen Xin, Chen Weiguan, Zuo Peng, Chen Weihai, Qiao Zhenguo, Fan Xingjuan
Department of Intensive Care Unit, Affiliated Haian Hospital of Nantong University Nantong 226600, Jiangsu, China.
Department of Neurology, Affiliated Hospital of Nantong University Nantong 226001, Jiangsu, China.
Int J Clin Exp Pathol. 2020 May 1;13(5):1136-1145. eCollection 2020.
As an evolutionarily conserved RNA-binding protein, LIN28 is known to be involved in the regulation of the translation and stability of a large number of mRNAs and the biogenesis of certain miRNAs. Increasing evidence indicates that LIN28 regulates many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, the expression and function of LIN28 after intracerebral hemorrhage (ICH) are still unclear. In this study, we performed an intracranial hemorrhage model in adult rats and western blot, immunohistochemistry, as well as immunofluorescence showed that LIN28 was obviously up-regulation in neurons adjacent to the hematoma after ICH. Besides, the transitory increase of LIN28 expression was paralleled with the up-regulation of proliferating cell nuclear antigen (PCNA) as well as GFAP. Hence, LIN28 might play an important role in astrocyte proliferation after ICH.
作为一种进化保守的RNA结合蛋白，已知LIN28参与大量mRNA的翻译和稳定性调控以及某些miRNA的生物合成。越来越多的证据表明，LIN28调节许多细胞过程，如胚胎干细胞增殖、细胞命运转变、发育时间调控和肿瘤发生。然而，脑出血（ICH）后LIN28的表达和功能仍不清楚。在本研究中，我们在成年大鼠中建立了颅内出血模型，蛋白质免疫印迹法、免疫组织化学以及免疫荧光显示，ICH后血肿周围神经元中LIN28明显上调。此外，LIN28表达的短暂增加与增殖细胞核抗原（PCNA）以及胶质纤维酸性蛋白（GFAP）的上调同时出现。因此，LIN28可能在ICH后星形胶质细胞增殖中起重要作用。
