Zhao Nannan, Sheng Minghao, Zhao Jie, Ma Xuelian, Wei Qiang, Song Qian, Zhang Kang, Xu Wenying, Sun Chuanqing, Liu Fengxia, Su Zhen
College of Biological Sciences, China Agricultural University, Beijing, China.
Beijing Key Laboratory of Crop, Ministry of Education (MOE) Laboratory of Crop Heterosis and Utilization, National Center for Evaluation of Agricultural Wild Plants (Rice), Beijing, China.
Front Bioeng Biotechnol. 2020 May 19;8:471. doi: 10.3389/fbioe.2020.00471. eCollection 2020.
Histone deacetylases (HDACs) influence chromatin state and gene expression. Eighteen genes with important biological functions have been identified in rice. In this study, we surveyed the gene presence frequency of all 18 rice genes in 3,010 rice accessions. showed insertion/deletion (InDel) polymorphisms in almost 98.8% accessions but only 1% accessions. InDel polymorphism association analysis showed that accessions with partial deletions in tended to display early leaf senescence. Further transgenic results confirmed that delayed leaf senescence in rice. The over-expression of delayed leaf senescence, and the knock-down of accelerated leaf senescence. Transcriptome analysis showed that photosynthesis and chlorophyll biosynthesis related genes were up-regulated in over-expression lines, while some programmed cell death and disease resistance related genes were down-regulated. Co-expression network analysis with gene expression view revealed that was co-expressed with multiple genes, particularly , which was significantly up-regulated in : lines. InDels in the promoter region of and in the gene region of occurred coincidentally among more than 90% accessions, and we identified multiple W-box motifs at the InDel position of . Over-expression of also delayed leaf senescence in rice. Taken together, our results suggest that both and are involved in regulating the process of leaf senescence in rice.
组蛋白去乙酰化酶（HDACs）影响染色质状态和基因表达。在水稻中已鉴定出18个具有重要生物学功能的基因。在本研究中，我们调查了3010份水稻种质中所有18个水稻基因的基因存在频率。结果显示，几乎98.8%的种质中存在插入/缺失（InDel）多态性，但只有1%的种质中存在。InDel多态性关联分析表明，在[具体基因]中存在部分缺失的种质往往表现出叶片早衰。进一步的转基因结果证实，[具体基因]延缓了水稻叶片衰老。[具体基因]的过表达延缓了叶片衰老，而[具体基因]的敲低则加速了叶片衰老。转录组分析表明，光合作用和叶绿素生物合成相关基因在[具体基因]过表达株系中上调，而一些程序性细胞死亡和抗病相关基因下调。通过基因表达视图进行的共表达网络分析表明，[具体基因]与多个基因共表达，特别是[具体基因]，在[具体基因]过表达株系中显著上调。[具体基因]启动子区域的InDels和[具体基因]基因区域的InDels在90%以上的种质中同时出现，并且我们在[具体基因]的InDel位置鉴定出多个W-box基序。[具体基因]的过表达也延缓了水稻叶片衰老。综上所述，我们的结果表明[具体基因]和[具体基因]都参与调控水稻叶片衰老过程。
