Goudarzi M, Razeghi M, Chirani A Salimi, Fazeli M, Tayebi Z, Pouriran R
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
New Microbes New Infect. 2020 May 15;36:100695. doi: 10.1016/j.nmni.2020.100695. eCollection 2020 Jul.
Methicillin-resistant (MRSA) strains that carry the gene are disseminated worldwide with varying regional incidences and different genetic backgrounds. The data on molecular characteristics of these strains is insufficient in Iran. The present study aimed to assess the characteristics and distribution of types of -positive MRSA strains. We investigated 89 MRSA isolates carrying the gene with typing, resistance gene detection and antimicrobial susceptibility. Of the 89 tested isolates, 61 (68.5%) were confirmed as multidrug resistant (MDR). The isolates were distributed across seven clonal complexes (CCs) including CC22 (42.7%), CC8 (28.1%), CC5 (11.2%), CC59 (5.6%), CC30 (4.5%), CC80 (4.5%) and CC15 (3.4%). typing identified 11 distinct types, with t223 (16.9%) and t790 (15.7%) being the most prevalent. All high-level mupirocin-resistant strains belonged to t002 ( = 8) and low-level mupirocin-resistant strains belonged to t790 ( = 6) types. Fusidic-acid-resistant isolates belonged to t437 ( = 3). iMLS phenotype was observed in t005 (6.7%), t002 (5.6%), t790 (3.4%), and t030, t044 and t084 (each 2.2%). It was found that in the -carrying MRSA strains, there were genetic diversities with a majority of the t223 type. Indeed, there is a necessity for more constructive surveillance/infection control strategies to address the prevalence and prevention of the emerging types.
携带该基因的耐甲氧西林金黄色葡萄球菌（MRSA）菌株在全球范围内传播，地区发病率各异，基因背景也不同。在伊朗，关于这些菌株分子特征的数据不足。本研究旨在评估携带该基因的MRSA菌株的特征和分布情况。我们通过多位点序列分型、耐药基因检测和抗菌药物敏感性试验，对89株携带该基因的MRSA分离株进行了研究。在89株受试分离株中，61株（68.5%）被确认为多重耐药（MDR）。这些分离株分布在7个克隆复合体（CCs）中，包括CC22（42.7%）、CC8（28.1%）、CC5（11.2%）、CC59（5.6%）、CC30（4.5%）、CC80（4.5%）和CC15（3.4%）。多位点序列分型鉴定出11种不同类型，其中t223（16.9%）和t790（15.7%）最为常见。所有对莫匹罗星高水平耐药的菌株均属于t002型（n = 8），对莫匹罗星低水平耐药的菌株属于t790型（n = 6）。对夫西地酸耐药的分离株属于t437型（n = 3）。在t005（6.7%）、t002（5.6%）、t790（3.4%）以及t030、t044和t084型（各2.2%）中观察到诱导型大环内酯-林可酰胺-链阳霉素B（iMLS）表型。研究发现，在携带该基因的MRSA菌株中存在遗传多样性，其中大多数为t223型。确实有必要采取更具建设性的监测/感染控制策略，以应对新出现类型菌株的流行和预防问题。
