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采用新研制的 CDs 重氮偶合分光光度法评价一些中式家常菜中亚硝酸盐含量的变化。

Evaluating nitrite content changes in some Chinese home cooking with a newely-developed CDs diazotization spectrophotometry.

机构信息

School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

出版信息

Food Chem. 2020 Nov 15;330:127151. doi: 10.1016/j.foodchem.2020.127151. Epub 2020 May 30.

Abstract

The research developed a diazo-coupling carbon-dots (CDs) method for determining nitrite and optimized variables of sodium sulfanilate, CDs synthesis, characteristic wavelength, reaction time and temperature. The method can assay 0.025-2.0 mg/L NO and has a detection limit of 9.6 μg/L and 95-105% recovery. Subsequently, it was applied in detecting NO changes in some Chinese home cooking, the gotten results indicated that if the sautéed vegetables (cabbage, Chinese cabbage, spinach and lettuce) and stir-fry pork, fried peas and pickled vegetable are stored at 4 °C for 72 h, nitrite contents are far lower than the recommended value, but if stored at room temperature for 24 h, the content in pure vegetables and shredded pork with green pepper will exceed the recommended value. Therefore, the staying fresher for the sautéed vegetables at room temperature is 24 h, if stored in a refrigerator at 4 °C, their staying fresher can be extended to 72 h.

摘要

研究开发了一种重氮偶联碳点(CDs)方法来测定亚硝酸盐,并优化了磺胺酸钠、CDs 合成、特征波长、反应时间和温度等变量。该方法可测定 0.025-2.0mg/L 的 NO,检测限为 9.6μg/L,回收率为 95-105%。随后,将其应用于检测一些中式家常菜中 NO 的变化,结果表明,如果将炒蔬菜(白菜、大白菜、菠菜和生菜)和炒猪肉、炒豌豆和腌菜在 4°C 下储存 72 小时,亚硝酸盐含量远低于推荐值,但如果在室温下储存 24 小时,纯蔬菜和青椒碎猪肉中的含量将超过推荐值。因此,炒蔬菜在室温下的保鲜时间为 24 小时,如果储存在 4°C 的冰箱中,其保鲜时间可以延长至 72 小时。

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