长链非编码 RNA DGCR5 通过影响 Wnt/β-catenin 信号通路促进三阴性乳腺癌的发生。

Long non-coding RNA DGCR5 incudes tumorigenesis of triple-negative breast cancer by affecting Wnt/β-catenin signaling pathway.

机构信息

Department of Breast Surgery, the First Affiliated Hospital, School of Medicine of Xi'an Jiaotong University, Xi'an 710061, China.

出版信息

J BUON. 2020 Mar-Apr;25(2):702-708.

PMID:32521856

Abstract

PURPOSE

Triple-negative breast cancer (TNBC) is one of the most ordinary malignant tumors. Recent studies have revealed that long noncoding RNAs (lncRNAs) play an important role in the progression of tumorigenesis. This study aimed to identify how lncRNA DGCR5 functions in the progression of TNBC.

METHODS

DGCR5 expression of both 57 paired TNBC patients' tissue samples and cells was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Moreover, the function of SNHG7 was identified by performing proliferation assay and transwell assay in vitro. Besides, the underlying mechanism was explored through Western blot assay and RT-qPCR. In addition, tumor formation and metastasis assays were also conducted in vivo.

RESULTS

In this study, DGCR5 expression was obviously higher in TNBC tissues when compared with that in adjacent non-tumor samples. Cell proliferation, migration and invasion in TNBC were inhibited after knockdown of DGCR5 in vitro. Moreover, results of further experiments revealed that the targeted proteins in Wnt/β-catenin signaling pathway were downregulated via knockdown of DGCR5 in TNBC. Furthermore, tumor formation and metastasis of TNBC were inhibited via knockdown of DGCR5 in nude mice.

CONCLUSIONS

Our study suggests that DGCR5 enhances TNBC cell proliferation and metastasis via inducing Wnt/β-catenin signaling pathway in vitro and in vivo.

摘要

目的

三阴性乳腺癌（TNBC）是最常见的恶性肿瘤之一。最近的研究表明，长非编码 RNA（lncRNA）在肿瘤发生发展中起着重要作用。本研究旨在探讨 lncRNA DGCR5 在 TNBC 进展中的作用机制。

方法

采用实时定量聚合酶链反应（RT-qPCR）检测 57 对 TNBC 患者组织样本和细胞中的 DGCR5 表达。此外，通过体外增殖试验和 Transwell 试验鉴定 SNHG7 的功能。通过 Western blot 试验和 RT-qPCR 探讨其潜在机制。另外，还进行了体内肿瘤形成和转移实验。

结果

本研究发现，与相邻非肿瘤样本相比，TNBC 组织中 DGCR5 的表达明显升高。体外敲低 DGCR5 可抑制 TNBC 细胞的增殖、迁移和侵袭。进一步的实验结果表明，敲低 DGCR5 可下调 Wnt/β-catenin 信号通路中的靶向蛋白。此外，在裸鼠体内敲低 DGCR5 可抑制 TNBC 的肿瘤形成和转移。