Department of Reproductive Genetics, Liaocheng People's Hospital, Liaocheng 252000, China.
J BUON. 2020 Mar-Apr;25(2):797-804.
Ovarian cancer (OC) is one of the most common malignancies in females with high mortality rate. MicroRNAs (miRNAs or miRs) serve as oncogenes or tumor suppressors in various human cancer types, including OC. The aim of this study was to explore the roles of miR-492 in OC.
Two human ovarian cancer cell lines, SKOV3 and CAOV3, and a normal ovarian cell line IOSE80 were used in this study. Real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to measure the mRNA levels of miRNAs and genes. The protein levels of epithelial-mesenchymal transition (EMT) associated genes were calculated using Western blot. Transwell assay was utilized to evaluate the migratory and invasive capacities.
MiR-492 was overexpressed while SRY-box 7 (SOX7) was lowly expressed in OC tissues and cells. Upregulation of miR-492 or downregulation of SOX7 predicted poor prognosis of OC patients. MiR-492 regulated the expression of SOX7 via directly binding to the 3'-untranslated region (3'-UTR) of SOX7 mRNA in SKOV3 OC cells. The expression of miR-492 had a negative relationship with SOX7 in OC tissues. MiR-492 promoted the migration, invasion and EMT through SOX7 in SKOV3 cells. SOX7 could partially reverse the role of miR-492 on the migratory, invasive and EMT abilities in SKOV3 cells.
MiR-492 promoted the migratory, invasive and EMT abilities through SOX7 in OC. This suggested that miR-492/SOX7 axis may be an effective candidate therapeutic target for the treatment of OC.
卵巢癌（OC）是女性中最常见的恶性肿瘤之一，死亡率很高。 microRNAs（miRNAs 或 miRs）在包括 OC 在内的多种人类癌症类型中充当癌基因或肿瘤抑制因子。本研究旨在探讨 miR-492 在 OC 中的作用。
本研究使用了两种人卵巢癌细胞系 SKOV3 和 CAOV3 以及正常卵巢细胞系 IOSE80。实时定量聚合酶链反应（RT-qPCR）用于测量 miRNA 和基因的 mRNA 水平。使用 Western blot 计算上皮-间充质转化（EMT）相关基因的蛋白水平。Transwell 测定用于评估迁移和侵袭能力。
miR-492 在 OC 组织和细胞中高表达，而 SRY-box 7（SOX7）低表达。miR-492 的上调或 SOX7 的下调预示着 OC 患者的预后不良。miR-492 通过直接结合 SOX7 mRNA 的 3'-非翻译区（3'-UTR）来调节 SKOV3 OC 细胞中 SOX7 的表达。miR-492 在 OC 组织中与 SOX7 的表达呈负相关。miR-492 通过 SOX7 促进 SKOV3 细胞的迁移、侵袭和 EMT。SOX7 可部分逆转 miR-492 对 SKOV3 细胞迁移、侵袭和 EMT 能力的作用。
miR-492 通过 SOX7 促进 OC 中的迁移、侵袭和 EMT 能力。这表明 miR-492/SOX7 轴可能是治疗 OC 的有效候选治疗靶点。
