Department of General Surgery, the third People's Hospital of Liaocheng, Liaocheng 252000, China.
J BUON. 2020 Mar-Apr;25(2):981-986.
To explore the effects of aspirin (ASP) on the proliferation and apoptosis of HepG2 hepatocellular carcinoma (HCC) cells via the Wnt/β-catenin signaling pathway.
Human HCC cells were cultured and treated with ASP at different concentrations. Cell proliferation was determined with cell counting kit-8 (CCK-8) and colony formation, and the rate of apoptosis was measured by flow cytometry. Western blotting (WB) and quantitative polymerase chain reaction (qRT-PCR) assays were used to assess the changes in the expression levels of related proteins.
ASP showed a time-and concentration-depented inhibitory effect on HepG2 cell proliferation. The number of colonies formed in ASP-treated HCC cells was significantly lower than in control cells. For HCC cells treated with ASP, the apoptosis rate enhanced with the increase of ASP concentration. The expression levels of TCF4 and LEF1, key molecules of the Wnt/β-catenin signaling pathway, were lowered in HCC cells treated with 4 mM ASP, and the nuclear translocation of β-catenin was weakened. The β-catenin activator exerted a negative influence on the anticancer effect of ASP.
ASP inhibits the proliferation and promotes the apoptosis of HCC cells through the Wnt/β-catenin signaling pathway.
探讨阿司匹林（ASP）通过 Wnt/β-连环蛋白信号通路对 HepG2 肝癌（HCC）细胞增殖和凋亡的影响。
培养人 HCC 细胞，并以不同浓度的 ASP 进行处理。采用细胞计数试剂盒-8（CCK-8）和集落形成实验检测细胞增殖，采用流式细胞术检测细胞凋亡率。Western blot（WB）和定量聚合酶链反应（qRT-PCR）检测相关蛋白表达水平的变化。
ASP 对 HepG2 细胞增殖表现出时间和浓度依赖性的抑制作用。ASP 处理的 HCC 细胞形成的集落数量明显少于对照组。随着 ASP 浓度的增加，ASP 处理的 HCC 细胞的凋亡率增加。Wnt/β-连环蛋白信号通路的关键分子 TCF4 和 LEF1 在接受 4 mM ASP 处理的 HCC 细胞中的表达水平降低，β-连环蛋白的核转位减弱。β-连环蛋白激活剂对 ASP 的抗癌作用产生负面影响。
ASP 通过 Wnt/β-连环蛋白信号通路抑制 HCC 细胞的增殖并促进其凋亡。
