重症肌无力患者体内是否存在针对抗乙酰胆碱受体抗体的自发性抗独特型抗体？
Are spontaneous anti-idiotypic antibodies against anti-acetylcholine receptor antibodies present in myasthenia gravis?
作者信息
Vincent A C
机构信息
Department of Neurological Sciences, Royal Free Hospital School of Medicine, London, UK.
出版信息
J Autoimmun. 1988 Apr;1(2):131-42. doi: 10.1016/0896-8411(88)90021-2.
The presence of anti-acetylcholine receptor anti-idiotypic antibodies in sera from 102 myasthenia gravis patients and from 33 first-degree relatives was investigated by: (a) Enzyme linked immunosorbent assay (ELISA) using monoclonal antibodies raised against human acetylcholine receptor, (b) immunoprecipitation of 125I-monoclonal anti-acetylcholine receptor antibodies; (c) inhibition of anti-acetylcholine receptor monoclonal antibody binding to the receptor and/or (d) inhibition of autologous and heterologous anti-acetylcholine receptor antibody binding to the receptor. No clear evidence for the presence of abnormal levels of spontaneous anti-idiotypic antibodies to anti-acetylcholine receptor antibodies was found.
通过以下方法对102例重症肌无力患者和33例一级亲属血清中抗乙酰胆碱受体抗独特型抗体的存在情况进行了研究：(a) 使用针对人乙酰胆碱受体产生的单克隆抗体进行酶联免疫吸附测定(ELISA)；(b) 对125I-单克隆抗乙酰胆碱受体抗体进行免疫沉淀；(c) 抑制抗乙酰胆碱受体单克隆抗体与受体的结合，和/或(d) 抑制自身和异源抗乙酰胆碱受体抗体与受体的结合。未发现存在异常水平的抗乙酰胆碱受体抗体自发抗独特型抗体的明确证据。
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