Sasaki K, Matsumura G
Department of Anatomy, Hokkadido University, School of Medicine, Sapporo, Japan.
J Anat. 1988 Oct;160:27-37.
Development of splenic haemopoiesis and morphology of lymphocytes in the embryonic and neonatal mice were examined by light and electron microscopy. At 15 days of gestation, free mononuclear cells were scattered throughout the splenic anlage, and the spleen was prehaemopoietic. At 16 days of gestation, immature erythroid cells and small lymphocytes first appeared and then the spleen became predominantly erythropoietic. In the red pulp, lymphocytes constituted approximately 2% of haemopoietic cells during fetal life and 3.6% at 4 days after birth. Splenic lymphocytes in the embryo had a nucleus 3.5-4.5 microns in diameter, and the majority were small lymphocytes with sparse and dark cytoplasm. Small lymphocytes occasionally had an invagination of the inner nuclear membrane into the nucleoplasm, showing various sectional profiles; in ultrathin sections, the proportion of small lymphocytes having the nuclear membrane invagination comprised 14.3% of the small lymphocytes in the embryonic spleen.
通过光学显微镜和电子显微镜检查胚胎和新生小鼠脾脏造血的发育及淋巴细胞的形态。妊娠15天时，游离单核细胞散布于整个脾脏原基，脾脏处于造血前期。妊娠16天时，未成熟红细胞和小淋巴细胞首次出现，随后脾脏主要进行红细胞生成。在红髓中，淋巴细胞在胎儿期约占造血细胞的2%，出生后4天占3.6%。胚胎期脾脏淋巴细胞的细胞核直径为3.5 - 4.5微米，大多数是小淋巴细胞，细胞质稀疏且深染。小淋巴细胞偶尔可见内核膜向核质内陷，呈现出各种切面形态；在超薄切片中，具有核膜内陷的小淋巴细胞占胚胎脾脏小淋巴细胞的14.3%。
