Wen Hailing, Xing Liang, Sun Kui, Xiao Changshuan, Meng Xiangxi, Yang Jingzhe
Department of Burns and Plastic Surgery, Affiliated Hospital of Chengde Medical University, Chengde, Hebei 067000, P.R. China.
Department of Burns and Plastic Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.
Exp Ther Med. 2020 Jul;20(1):591-598. doi: 10.3892/etm.2020.8725. Epub 2020 May 7.
Severe burns may lead to intestinal inflammation and oxidative stress, resulting in intestinal barrier damage and gut dysfunction. Loganin, an iridoid glycoside compound, has been isolated from Sieb. et Zucc; however, its role in the treatment of burn injury is yet to be fully elucidated. Therefore, the present study examined the effect of loganin administration on burn-induced intestinal inflammation and oxidative stress after severe burns in male Sprague-Dawley rats. Histological injury was assessed by hematoxylin and eosin staining. Furthermore, cytokine expression in intestinal tissues was measured by ELISA and reverse transcription-quantitative PCR. Antioxidative activities were assessed by determining the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). Apoptosis was detected by flow cytometry. Apoptosis-related proteins, toll-like receptor 4 (TLR4) protein and NF-κB translocation were examined by western blotting. Immunohistochemical staining was used to observe TLR4 and NF-κB p65 expression in intestinal tissues. The present study suggested that loganin administration significantly reduced burn injury-induced intestinal histological changes, tumor necrosis factor-α, interleukin (IL)-6 and IL-1β production and oxidative stress, evidenced by decreased ROS levels and MDA content (P<0.05). Furthermore, loganin increased SOD, CAT and GSH-Px levels and intestinal epithelial cell apoptosis. Loganin treatment also significantly inhibited activation of the TLR4/NF-κB signaling pathway in the intestine of severely burned rats (P<0.05). In conclusion, loganin reduced burns-induced intestinal inflammation and oxidative stress, potentially by regulating the TLR4/NF-κB signaling pathway.
严重烧伤可能导致肠道炎症和氧化应激，进而引起肠屏障损伤和肠道功能障碍。马钱苷，一种环烯醚萜苷类化合物，已从 中分离得到；然而，其在烧伤治疗中的作用尚未完全阐明。因此，本研究检测了给予马钱苷对雄性Sprague-Dawley大鼠严重烧伤后烧伤诱导的肠道炎症和氧化应激的影响。通过苏木精和伊红染色评估组织学损伤。此外，通过酶联免疫吸附测定法和逆转录-定量聚合酶链反应测量肠道组织中的细胞因子表达。通过测定活性氧（ROS）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）的水平评估抗氧化活性。通过流式细胞术检测细胞凋亡。通过蛋白质免疫印迹法检测凋亡相关蛋白、Toll样受体4（TLR4）蛋白和核因子κB（NF-κB）易位。免疫组织化学染色用于观察肠道组织中TLR4和NF-κB p65的表达。本研究表明，给予马钱苷可显著减轻烧伤诱导的肠道组织学变化、肿瘤坏死因子-α、白细胞介素（IL）-6和IL-1β的产生以及氧化应激，表现为ROS水平和MDA含量降低（P<0.05）。此外，马钱苷可提高SOD、CAT和GSH-Px水平并增加肠上皮细胞凋亡。马钱苷治疗还可显著抑制严重烧伤大鼠肠道中TLR4/NF-κB信号通路的激活（P<0.05）。总之，马钱苷可能通过调节TLR4/NF-κB信号通路减轻烧伤诱导的肠道炎症和氧化应激。
