Department of Bone and Joint, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Department of Biological Sciences, Wichita State University, Wichita, KS 67260, USA.
Mol Med Rep. 2017 Oct;16(4):5555-5560. doi: 10.3892/mmr.2017.7292. Epub 2017 Aug 21.
Scutellarin is the major effective constituent of the commonly used Chinese medicine Erigeron breviscapus. It has been applied in the clinic to treat various diseases, and is characterized by high content, a stable source, controllable quality, high efficiency and low toxicity. In addition, its potential pharmacological effects have been increasingly identified and elucidated. The present study was performed to examine the role of scutellarin on collagen‑induced arthritis (CIA). Mice were injected subcutaneously with bovine collagen type II and administered scutellarin for 2 weeks by gavage 20 mg/kg/day. ELISA kits were used to measure the levels of interleukin (IL)‑1β, IL‑6, tumor necrosis factor‑α (TNF‑α), oxidative stress markers [superoxide dismutase (SOD) and malondialdehyde (MDA)] and caspase‑3/-9 activity. Bax, Bcl‑2, toll like receptor 4 (TLR4) and nuclear factor (NF)‑κB protein expression was analyzed using western immunoblot analyses. The present study demonstrated that scutellarin prevented CIA, and inhibited the expression of inflammation factors, IL‑1β, IL‑6 and TNF‑α. In addition, scutellarin reduced the levels of oxidative stress markers, SOD and MDA, as well as intercellular adhesion molecule‑1 and monocyte chemoattractant protein 1 in CIA mice. Caspase‑3/-9, Bax/Bcl‑2, TLR4 and NF‑κB protein expression were reduced in CIA mice following scutellarin treatment. The results of the current study suggest a novel effect of scutellarin involving the inhibition of TLR4/NF‑κB‑mediated inflammation.
野黄芩苷是常用中药灯盏花中的主要有效成分。它已在临床上用于治疗各种疾病，具有含量高、来源稳定、质量可控、高效低毒的特点。此外，其潜在的药理作用也越来越被认识和阐明。本研究旨在探讨野黄芩苷对胶原诱导性关节炎（CIA）的作用。小鼠皮下注射牛Ⅱ型胶原，灌胃给予野黄芩苷 20mg/kg/d，共 2 周。采用 ELISA 试剂盒检测白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）、氧化应激标志物[超氧化物歧化酶（SOD）和丙二醛（MDA）]和半胱氨酸天冬氨酸蛋白酶-3/-9（caspase-3/-9）活性。采用 Western 免疫印迹分析检测 Bax、Bcl-2、Toll 样受体 4（TLR4）和核因子（NF）-κB 蛋白表达。本研究表明，野黄芩苷可预防 CIA，并抑制炎症因子 IL-1β、IL-6 和 TNF-α的表达。此外，野黄芩苷可降低 CIA 小鼠氧化应激标志物 SOD 和 MDA 以及细胞间黏附分子-1 和单核细胞趋化蛋白 1 的水平。CIA 小鼠经野黄芩苷治疗后 caspase-3/-9、Bax/Bcl-2、TLR4 和 NF-κB 蛋白表达降低。本研究结果提示野黄芩苷具有通过抑制 TLR4/NF-κB 介导的炎症的新作用。
