Applied Biosafety Research Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada.
Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada.
BMC Res Notes. 2020 Jun 15;13(1):291. doi: 10.1186/s13104-020-05137-8.
Mouse models have delivered variable recapitulation of Kyasanur Forest disease (KFD) pathology and consistently demonstrated neurological involvement which may be a limited feature of human disease. With the purpose of more accurately modelling human disease progression we infected several small-mammalian models: guinea pigs, hamsters and ferrets with a titered infectious dose of Kyasanur Forest disease virus (KFDV). Clinical indicators of disease severity were observed for seventeen days, on day eighteen a visual post-mortem analysis of visceral organs was conducted. Viral load in selected tissues was measured to infer disease signs and the establishment of viral replication.
Daily monitoring did not reveal any observable signs of illness; weight loss was minimal across species and gross pathology did not indicate severe viral infection. Tissue specific tropism and establishment of viral infection was monitored by quantitative real-time polymerase chain reaction (qRT-PCR). No viral replication was detected in ferrets (n = 0/3), but was present in the spleen of guinea pigs (n = 3/3) and the brain of hamsters (n = 3/3). Low levels of viral RNA were detected in multiple hamster tissues (kidney, liver, lung and spleen) suggesting the possibility of viral tropism and possible adaptation to the host. No serological tests were performed.
鼠类模型对基孔肯雅热（KFD）病理学的再现存在差异，且一致表现出神经系统受累，这可能是人类疾病的一个有限特征。为了更准确地模拟人类疾病进展，我们用经滴定的基孔肯雅森林病毒（KFDV）感染了几种小型哺乳动物模型：豚鼠、仓鼠和雪貂。在十七天内观察疾病严重程度的临床指标，在第十八天对内脏器官进行视觉尸检分析。测量选定组织中的病毒载量，以推断疾病迹象和病毒复制的建立。
每日监测未发现任何可观察到的疾病迹象；体重减轻在各物种中均微不足道，大体病理学也未表明存在严重的病毒感染。通过实时定量聚合酶链反应（qRT-PCR）监测组织特异性嗜性和病毒感染的建立。在雪貂（n=0/3）中未检测到病毒复制，但在豚鼠的脾脏（n=3/3）和仓鼠的大脑（n=3/3）中存在病毒感染。在多种仓鼠组织（肾脏、肝脏、肺和脾脏）中检测到低水平的病毒 RNA，这表明可能存在病毒嗜性和可能对宿主的适应。未进行血清学检测。
