Department of Obstetrics and Gynecology, Chi-Mei Medical Center, Tainan, Taiwan; Department of Medicine, Taipei Medical University, Taipei, Taiwan; Department of Sport Management, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
Department of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center, Taipei, Taiwan.
Mol Cell Endocrinol. 2020 Aug 20;514:110915. doi: 10.1016/j.mce.2020.110915. Epub 2020 Jun 12.
Irisin has gained attention because of its potential applications in the treatment of metabolic diseases. Accumulating evidence indicates that irisin attenuates obesity via the browning of white adipose tissue; however, the underlying mechanisms are unclear. Here, we evaluated the effects of irisin on adipocyte browning and the underlying mechanisms. The western blotting and immunofluorescence analyses demonstrated that irisin significantly induced the up-regulation of brown fat-specific proteins (PGC1α, PRDM16, and UCP-1) and HO-1 in 3T3-L1 adipocytes. Moreover, irisin significantly increased the levels of cytosolic p62 and nuclear Nrf2. These effects of irisin in the adipocytes were attenuated by treatment with SnPP or p62 siRNA. In addition, the browning effect of irisin was observed in BAT-WT-1 cells. These findings suggest that irisin induced browning effect via the p62/Nrf2/HO-1 signalling pathway and that it may be a potential candidate for preventing or treating obesity.
鸢尾素因其在治疗代谢性疾病方面的潜在应用而受到关注。越来越多的证据表明，鸢尾素通过白色脂肪组织的褐变来减轻肥胖；然而，其潜在的机制尚不清楚。在这里，我们评估了鸢尾素对脂肪细胞褐变的影响及其潜在的机制。Western blot 和免疫荧光分析表明，鸢尾素显著诱导了 3T3-L1 脂肪细胞中棕色脂肪特异性蛋白（PGC1α、PRDM16 和 UCP-1）和 HO-1 的上调。此外，鸢尾素还显著增加了细胞质 p62 和核 Nrf2 的水平。用 SnPP 或 p62 siRNA 处理可减弱鸢尾素对脂肪细胞的这些作用。此外，在 BAT-WT-1 细胞中观察到了鸢尾素的褐变效应。这些发现表明，鸢尾素通过 p62/Nrf2/HO-1 信号通路诱导褐变效应，它可能是预防或治疗肥胖的潜在候选药物。
