一种用于实时食品样品、活组织和斑马鱼中甲醛比色检测的新型荧光探针。
A novel fluorescent probe for ratiometric detection of formaldehyde in real food samples, living tissues and zebrafish.
机构信息
Hunan Key Laboratory of Processed Food for Special Medical Purpose, National Engineering Laboratory for Deep Process of Rice and Byproducts, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
Hunan Key Laboratory of Processed Food for Special Medical Purpose, National Engineering Laboratory for Deep Process of Rice and Byproducts, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
出版信息
Food Chem. 2020 Nov 30;331:127221. doi: 10.1016/j.foodchem.2020.127221. Epub 2020 Jun 9.
Herein, a two-photon (TP) ratiometric fluorescent probe (NpFA) was developed for detecting formaldehyde (FA) in real food samples, living onion tissues and zebrafish by fluorescence resonance energy transfer (FRET) strategy. Specifically, a TP fluorophore as the donor and a FA turn-on naphthalimide fluorophore as the acceptor were connected by a non-conjugated linker to construct the TP-FRET-based NpFA, which exhibited a target-modulated ratiometric fluorescence response to FA rapidly with high selectivity and sensitivity during 65 s, and a large ratio ~5-fold enhancement at I/I after addition of FA, displaying ~60-fold enhancement at 550 nm and a quite low DOC of 5.8 ± 0.2 nM. Moreover, NpFA has a good imaging resolution and depth of deep tissue penetration. Therefore, based on the above results, NpFA has the capability to be a useful tool for investigating FA in real samples application, and we also hope NpFA will further study of the physiological and pathological function of FA.
本文开发了一种双光子（TP）比率荧光探针（NpFA），通过荧光共振能量转移（FRET）策略，用于检测实际食品样品、活体洋葱组织和斑马鱼中的甲醛（FA）。具体来说，将 TP 荧光团作为供体，将 FA 开启的萘酰亚胺荧光团作为受体，通过非共轭连接子连接，构建基于 TP-FRET 的 NpFA，该探针对 FA 表现出快速、高选择性和高灵敏度的目标调制比率荧光响应，在添加 FA 后，I/I 的比值增加约 5 倍，在 550nm 处的增强约 60 倍，DOC 低至 5.8±0.2nM。此外，NpFA 具有良好的成像分辨率和深层组织穿透深度。因此，基于上述结果，NpFA 有能力成为实际样品应用中研究 FA 的有用工具，我们也希望 NpFA 将进一步研究 FA 的生理和病理功能。
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