Ding Haiyuan, Yuan Gangqiang, Peng Longpeng, Zhou Liyi, Lin Qinlu
Hunan Key Laboratory of Processed Food for Special Medical Purpose, National Engineering Laboratory for Deep Process of Rice and Byproducts, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
J Agric Food Chem. 2020 Mar 18;68(11):3670-3677. doi: 10.1021/acs.jafc.9b08114. Epub 2020 Mar 9.
Formaldehyde (FA, HCHO) is a highly reactive carbonyl species, which is very harmful to humans and the environment as a tissue fixative and preservative. Therefore, developing some highly sensitive, selective, and rapid detection methods is significant for human health in food safety and environmental protection. Herein, a two-photon (TP) ratiometric sensor, , has been constructed by conjugating a TP donor (Π-push-pull-structure) with a FA off-on acceptor (functioned with hydrazide moiety) via a nonconjugated linker through the fluorescence resonance energy transfer mechanism. Such a scaffold affords a reliable and specific probe for detecting FA with two well-resolved emission peaks separated by 124 nm. Also, it responds to FA rapidly with high selectivity and sensitivity during 1.0 min and a large ratio enhancement at / with addition of 0-20μM FA, exhibiting ∼4-fold ratio increase and a fairly low LOD of 8.3 ± 0.3 nM. Moreover, has been successfully employed for detecting FA in live cells, onion tissues, and zebrafish, exhibiting that can serve as a useful tool for investigating FA in real food application and offering strong theoretical support and technical means for investigation of physiological and pathological functions of FA.
甲醛（FA，HCHO）是一种高反应性羰基化合物，作为一种组织固定剂和防腐剂，对人类和环境非常有害。因此，开发一些高灵敏度、高选择性和快速的检测方法对于食品安全和环境保护中的人类健康具有重要意义。在此，通过荧光共振能量转移机制，将双光子（TP）供体（Π-推-拉结构）与FA开-关受体（通过酰肼部分起作用）通过非共轭连接子共轭，构建了一种双光子（TP）比率传感器。这种支架提供了一种可靠且特异的探针，用于检测具有两个分辨率良好、间隔为124 nm的发射峰的FA。此外，它在1.0分钟内对FA快速响应，具有高选择性和灵敏度，在加入0-20μM FA时在/处有大的比率增强，显示出约4倍的比率增加和相当低的8.3±0.3 nM检测限。此外，已成功用于检测活细胞、洋葱组织和斑马鱼中的FA，表明可作为研究实际食品应用中FA的有用工具，并为研究FA的生理和病理功能提供有力的理论支持和技术手段。
