Li Chunting, Wang Jiexuan, Lu Xinmiao, Ge Huang, Jin Xin, Guan Qinhua, Su Yue, Pan Ruijun, Li Peiyong, Cai Wei, Zhu Xinyuan
Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, People's Republic of China.
Department of General Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, People's Republic of China; Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Biomaterials. 2020 Oct;255:120071. doi: 10.1016/j.biomaterials.2020.120071. Epub 2020 Jun 8.
Circulating tumor cells (CTCs) represent the most common way of tumor metastasis and has been considered as a significant index for tumor diagnosis, staging and prognosis. However, CTC detection and analysis are always limited by the scarcity of CTC in the peripheral blood and the interference of blood cells. Therefore, here we presented with a hydrogen peroxide (HO)-response nanoprobes with CD44-targeted ability to reduce the interference of blood cells and improve the detection efficiency and accuracy and the pancreatic cancer cell was used to evaluate the feasibility of our probe. Shortly, hydrophobic HO-response naphthalimide-borate fluorophore was introduced onto the hydrophilic hyaluronic acid to form an amphiphilic complex, which could self-assemble into fluorescent nanoprobes in water. Our studies demonstrated that the nanoprobes were not only able to specifically recognize the pancreatic cancer cells with overexpressed CD44 proteins and reduce the influence of white blood cells in the peripheral blood, but also capable of semi-quantifying HO content in CTCs, Which could be further used as a significant index for tumor clinical evaluation and therapy.
循环肿瘤细胞（CTCs）是肿瘤转移最常见的途径，一直被视为肿瘤诊断、分期和预后的重要指标。然而，CTCs的检测和分析总是受到外周血中CTCs数量稀少以及血细胞干扰的限制。因此，我们在此展示了一种具有靶向CD44能力的过氧化氢（HO）响应纳米探针，以减少血细胞的干扰，提高检测效率和准确性，并使用胰腺癌细胞评估我们探针的可行性。简而言之，将疏水性HO响应萘二甲酰亚胺 - 硼酸盐荧光团引入亲水性透明质酸上，形成两亲性复合物，该复合物可在水中自组装成荧光纳米探针。我们的研究表明，该纳米探针不仅能够特异性识别CD44蛋白过表达的胰腺癌细胞并减少外周血中白细胞的影响，还能够对CTCs中的HO含量进行半定量，这可进一步用作肿瘤临床评估和治疗的重要指标。
