Rawlings A V, Deegan T
Cardiothoracic Unit, Broadgreen Hospital, Liverpool, UK.
Ann Clin Biochem. 1988 Nov;25 ( Pt 6):638-44. doi: 10.1177/000456328802500607.
Lipoproteins of density, d less than 1.063, isolated by polyanion-cation precipitation methods, gave isoelectric focussing patterns of apolipoprotein E isoforms by rod-gel electrophoresis which differed from the corresponding patterns obtained from ultracentrifugally-derived very low density lipoproteins. The differences were sporadic and variable but the most common effect was an increased frequency of the E3 isoform. Two-dimensional analyses involving sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoelectrophoresis against anti-apolipoprotein A-I indicated that contamination of precipitated lipoproteins with pro-apolipoprotein A-I was responsible for this phenomenon. It is suggested that two-dimensional techniques should be applied for definitive phenotyping if precipitated lipoproteins are used as source material.
通过聚阴离子-阳离子沉淀法分离得到的密度小于1.063的脂蛋白，经棒状凝胶电泳进行载脂蛋白E亚型的等电聚焦分析，其图谱与超速离心法获得的极低密度脂蛋白的相应图谱不同。差异是零星且多变的，但最常见的影响是E3亚型的频率增加。涉及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和抗载脂蛋白A-I免疫电泳的二维分析表明，沉淀脂蛋白被载脂蛋白A-I原污染是造成这一现象的原因。建议如果使用沉淀脂蛋白作为源材料，应采用二维技术进行明确的表型分析。
