Kane J W, Gowland E
Ann Clin Biochem. 1986 Sep;23 ( Pt 5):509-13. doi: 10.1177/000456328602300504.
A method is described for the identification of apolipoprotein E phenotypes. Lipoproteins were precipitated by magnesium chloride/phosphotungstate reagent and isoelectric focusing performed on flat-bed agarose. Sensitivity was enhanced by blotting with nitrocellulose prior to staining. The system described requires no ultracentrifugation step and allows up to 20 samples to be processed in each run. Agarose has some advantages over the more commonly used polyacrylamide gels for isoelectric focusing.
本文描述了一种鉴定载脂蛋白E表型的方法。用氯化镁/磷钨酸盐试剂沉淀脂蛋白，并在平板琼脂糖上进行等电聚焦。在染色前用硝酸纤维素膜进行印迹可提高灵敏度。所述系统无需超速离心步骤，每次运行可处理多达20个样本。在等电聚焦方面，琼脂糖比更常用的聚丙烯酰胺凝胶具有一些优势。
