Supercomputing Facility for Bioinformatics & Computational Biology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India, 110016.
Centre of Evolution and Medicine, Arizona State University, Life Sciences C, 427 East Tyler Mall, Tempe, AZ 85281, United States.
Database (Oxford). 2020 Jan 1;2020. doi: 10.1093/database/baaa036.
Despite Plasmodium vivax being the main offender in the majority of malarial infections, very little information is available about its adaptation and development in humans. Its capability for activating relapsing infections through its dormant liver stage and resistance to antimalarial drugs makes it as one of the major challenges in eradicating malaria. Noting the immediate necessity for the availability of a comprehensive and reliable structural and functional repository for P. vivax proteome, here we developed a web resource for the new reference genome, PvP01, furnishing information on sequence, structure, functions, active sites and metabolic pathways compiled and predicted using some of the state-of-the-art methods in respective fields. The PvP01 web resource comprises organized data on the soluble proteome consisting of 3664 proteins in blood and liver stages of malarial cycle. The current public resources represent only 163 proteins of soluble proteome of PvP01, with complete information about their molecular function, biological process and cellular components. Also, only 46 proteins of P. vivax have experimentally determined structures. In this milieu of extreme scarcity of structural and functional information, PvP01 web resource offers meticulously validated structures of 3664 soluble proteins. The sequence and structure-based functional characterization led to a quantum leap from 163 proteins available presently to whole soluble proteome offered through PvP01 web resource. We believe PvP01 web resource will serve the researchers in identifying novel protein drug targets and in accelerating the development of structure-based new drug candidates to combat malaria. Database Availability: http://www.scfbio-iitd.res.in/PvP01.
尽管间日疟原虫是大多数疟疾感染的主要病原体，但关于它在人类体内的适应和发展的信息却很少。它通过休眠的肝脏阶段引发复发感染的能力以及对抗疟药物的耐药性，使其成为消除疟疾的主要挑战之一。鉴于迫切需要一个全面可靠的间日疟原虫蛋白质组结构和功能资源库，我们在这里开发了一个针对新参考基因组 PvP01 的网络资源，提供了关于序列、结构、功能、活性位点和代谢途径的信息，这些信息是使用各自领域的一些最先进的方法编译和预测的。PvP01 网络资源包括疟原虫生命周期的血液和肝脏阶段的 3664 种可溶性蛋白质组的组织化数据。目前的公共资源仅代表 PvP01 可溶性蛋白质组的 163 种蛋白质，具有关于它们的分子功能、生物过程和细胞成分的完整信息。此外，只有 46 种间日疟原虫蛋白具有实验确定的结构。在这种结构和功能信息极度匮乏的情况下，PvP01 网络资源提供了 3664 种可溶性蛋白质的精心验证结构。基于序列和结构的功能特征分析，使我们能够从目前可用的 163 种蛋白质飞跃到通过 PvP01 网络资源提供的整个可溶性蛋白质组。我们相信，PvP01 网络资源将有助于研究人员识别新型蛋白质药物靶标，并加速基于结构的新型药物候选物的开发，以对抗疟疾。数据库可用性：http://www.scfbio-iitd.res.in/PvP01。
