Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, No. 109 Xueyuan Xi Road Wenzhou, Zhejiang, 325027, China.
Cell Tissue Res. 2020 Sep;381(3):543-554. doi: 10.1007/s00441-020-03221-w. Epub 2020 Jun 15.
To explore the effects of aquaporin (AQP) 1 on pregnancy outcome and the association between expression of AQP1 and other AQPs in the placenta and foetal membranes, the rate of copulatory plugs and pregnancy, amniotic fluid (AF) volume, osmolality and composition were determined in AQP1-knockout (AQP1) mice at different gestational days (GD). The expression and location of AQP1 and other AQPs in the placenta and foetal membranes of AQP1 mice, AQP1-siRNA transfected WISH cells and oligohydramnios patients were also detected. Compared to control mice, AQP1 mice exhibited reduced copulation plug and successful pregnancy rates, but these effects were accompanied by a larger AF volume and lower AF osmolality at late gestation. AQP9 expression was significantly decreased in the placenta and foetal membranes of AQP1 mice, while AQP8 level was elevated in the foetal membranes of AQP1 mice. Moreover, AQP9 expression was suppressed in WISH cells after AQP1 downregulation. Furthermore, AQP9 expression was associated with AQP1 level in the placenta and foetal membranes in oligohydramnios. AQP1 may play a critical role in regulating pregnancy outcome and maternal-foetal fluid homeostasis. Changes in AQP1 expression may lead to compensatory alterations in AQP8 and AQP9 expression in the placenta.
为了探索水通道蛋白 1（AQP1）对妊娠结局的影响，以及 AQP1 在胎盘和胎膜中的表达与其他 AQPs 的关系，我们在不同妊娠天数（GD）检测了 AQP1 敲除（AQP1）小鼠的交配栓率和妊娠率、羊水（AF）体积、渗透压和成分。还检测了 AQP1 小鼠、转染 AQP1-siRNA 的 WISH 细胞和羊水过少患者的胎盘和胎膜中 AQP1 和其他 AQPs 的表达和定位。与对照组小鼠相比，AQP1 小鼠的交配栓和成功妊娠率降低，但这些影响伴随着晚期妊娠时羊水体积增大和羊水渗透压降低。AQP1 小鼠的胎盘和胎膜中 AQP9 表达明显降低，而 AQP8 水平在 AQP1 小鼠的胎膜中升高。此外，AQP1 下调后 WISH 细胞中的 AQP9 表达受到抑制。此外，羊水过少患者的胎盘和胎膜中 AQP9 的表达与 AQP1 水平相关。AQP1 可能在调节妊娠结局和母胎液体平衡中发挥关键作用。AQP1 表达的变化可能导致胎盘和胎膜中 AQP8 和 AQP9 表达的代偿性改变。
