Azarabadi Mahdi, Heidari Fatemeh, Khaki Amir Afshin, Kaka Gholamreza, Ghadian Alireza
Nephrology and Urology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Obstetrics and Gynecology, Universitätsklinikum Schleswig-Holstein Campus Lübeck, Lübeck, Germany.
Andrologia. 2020 Oct;52(9):e13704. doi: 10.1111/and.13704. Epub 2020 Jun 15.
Testicular torsion is a serious urological disease leading to testicular damage. This study aimed to assess the effect of minocycline on testicular ischaemia/reperfusion (I/R) injury caused by testicular torsion/detorsion. Male adult Wistar rats (n = 32) were assigned into four groups of sham, I/R, I/R + minocycline and minocycline. I/R injury was induced by two sets of surgical operations, including the rotation of the left testis (720°, counterclockwise), followed by detorsion after 4 hr. The administration of minocycline was carried out 30 min before detorsion and then continued for 8 weeks. At the end of the 8th week, rats were killed and sampling was done. Johnson's score, the height of seminiferous tubule epithelium, the mean seminiferous tubule diameter, as well as biochemical parameters, SOD, GPx and CAT, were significantly enhanced in the I/R + minocycline group compared with the I/R group. The administration of minocycline led to a marked decrease in expression levels of Caspase-3, Bax, IL-1β and TNF-α genes, and a remarkable increase in expression levels of Bcl-2, 3β-HSD and 17β-HSD3 genes compared with the I/R group. Administration of minocycline could also reduce the rate of germ cell apoptosis (TUNEL staining). Hence, minocycline was useful in the management of testicular torsion/detorsion.
睾丸扭转是一种导致睾丸损伤的严重泌尿系统疾病。本研究旨在评估米诺环素对睾丸扭转/复位所致睾丸缺血/再灌注（I/R）损伤的影响。将成年雄性Wistar大鼠（n = 32）分为四组：假手术组、I/R组、I/R + 米诺环素组和米诺环素组。通过两组外科手术诱导I/R损伤，包括将左侧睾丸逆时针旋转720°，4小时后再复位。在复位前30分钟给予米诺环素，并持续给药8周。在第8周结束时，处死大鼠并进行取样。与I/R组相比，I/R + 米诺环素组的约翰逊评分、生精小管上皮高度、生精小管平均直径以及生化参数超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）均显著升高。与I/R组相比，给予米诺环素导致半胱天冬酶-3（Caspase-3）、Bax、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）基因表达水平显著降低，而Bcl-2、3β-羟基类固醇脱氢酶（3β-HSD）和17β-羟基类固醇脱氢酶3（17β-HSD3）基因表达水平显著升高。给予米诺环素还可降低生殖细胞凋亡率（TUNEL染色）。因此，米诺环素对睾丸扭转/复位的治疗有效。
