基于 I-III 期直肠癌复发相关差异剪接的整合分析预测复发的标志物。
A signature predicting relapse based on integrated analysis on relapse-associated alternative mRNA splicing in I-III rectal cancer.
机构信息
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
出版信息
Genomics. 2020 Sep;112(5):3274-3283. doi: 10.1016/j.ygeno.2020.06.021. Epub 2020 Jun 13.
Researches focusing on the effects of alternative splicing (AS) on relapse of rectal cancer is little and signature based on the AS is blank. In this study, bioinformatic analysis was performed to identify and analyze the relapse-associated ASs, a signature was also constructed. In conclusion, 829 relapse-associated ASs of 676 mRNA were identified. 603 proteins with 2119 interactions were involved in the PPI (protein-protein interactions) network. 43 relapse-associated ASs and 64 SFs (splicing factors) with 160 interactions were indicated. Finally, we built a robust signature to predict the relapse of I-III rectal cancer with a high AUC (0.98) of ROC at 1 year. Based on the ASs involved in the signature, 4 molecular subgroups that could distinguish the relapse rate in diverse groups were identified. Our research provided an overview of relapse-associated ASs in I-III rectal cancer.
针对直肠癌复发的选择性剪接 (AS) 影响的研究很少，基于 AS 的特征也是空白的。在这项研究中，进行了生物信息学分析，以识别和分析与复发相关的 AS，并构建了一个特征。总之，鉴定出 676 个 mRNA 中有 829 个与复发相关的 AS。PPI（蛋白质-蛋白质相互作用）网络中涉及 603 种蛋白质和 2119 种相互作用。有 43 个与复发相关的 AS 和 64 个 SF（剪接因子），有 160 种相互作用。最后，我们构建了一个稳健的特征来预测 I-III 期直肠癌的复发，ROC 在 1 年内的 AUC（0.98）很高。基于特征中涉及的 AS，确定了 4 个可以区分不同组复发率的分子亚组。我们的研究提供了 I-III 期直肠癌中与复发相关的 AS 的概述。
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