Sabancı University, Faculty of Engineering and Natural Sciences, Department of Materials Science and Nanoengineering, 34956 İstanbul, Turkey.
Yıldız Technical University, Graduate School of Natural and Applied Science, Department of Bioengineering, 34220 İstanbul, Turkey.
Int J Biol Macromol. 2020 Oct 15;161:947-957. doi: 10.1016/j.ijbiomac.2020.06.066. Epub 2020 Jun 13.
Epilobium is a medicinal plant; its extracts are widely used traditional medicine due to their broad range of pharmacological and therapeutic properties. Its most prominent feature is its therapeutic effects on prostatic diseases. The aim of this study is preparation of controlled release system of Epilobium parviflorum, and determination of its potential of anticancer applications. For this purpose, Epilobium parviflorum extract (EPE) loaded chitosan nanoparticles were prepared with ionic gelation method to increase the bioavailability of the extract. The nanoparticles were investigated in terms of size, zeta potential, polydispersity index, encapsulation efficiency, loading capacity and release profile. Besides, scanning electron microscopy (SEM) was used to observe the morphology of the nanoparticles. Moreover, Ames/Salmonella test was used to determine the mutagenicity of EPE, and it was shown that it had no mutagenic effect. It was found that EPE loaded chitosan nanoparticles were with 64.47 nm in average size, 0.168 PdI and 15.2 mV zeta potential. Encapsulation efficiency and loading capacity were found as 92.46% and 8%, respectively. Finally, DNA binding assay and in silico molecular docking studies were performed between EPE and DNA in order to contribute to design of plant based controlled release system for use in cancer therapy.
柳叶菜是一种药用植物，其提取物由于具有广泛的药理和治疗特性，被广泛应用于传统医学。它最显著的特点是对前列腺疾病有治疗作用。本研究旨在制备柳兰花提取物（EPE）的控释系统，并确定其在抗癌应用中的潜力。为此，采用离子凝胶法制备了载有 EPE 的壳聚糖纳米粒，以提高提取物的生物利用度。对纳米粒的粒径、Zeta 电位、多分散指数、包封率、载药量和释放特性进行了研究。此外，还使用扫描电子显微镜（SEM）观察了纳米粒的形态。此外，还采用艾姆斯/沙门氏菌试验法测定了 EPE 的致突变性，结果表明其无致突变作用。结果表明，载有 EPE 的壳聚糖纳米粒的平均粒径为 64.47nm，PdI 为 0.168，Zeta 电位为 15.2mV。包封率和载药量分别为 92.46%和 8%。最后，进行了 DNA 结合实验和 EPE 与 DNA 之间的计算机分子对接研究，为设计基于植物的控释系统用于癌症治疗提供了依据。
