Center for Clinical Pharmacology, Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju, Republic of Korea.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Obes Facts. 2020;13(3):375-385. doi: 10.1159/000507817. Epub 2020 Jun 16.
Gene expression profiles in human peripheral blood mononuclear cells (PBMCs) may act as a useful tool to better understand obesity. We investigated gene expression levels in PMBCs for possible differences between obese and non-obese subjects (19-55 years) and evaluated correlations between gene expression in PBMCs and clinical obesity indices.
Body weight, BMI, fat amount, fat percentage, waist/hip ratio, leptin, and adiponectin levels were determined in 30 obese and 20 non-obese subjects. Expression levels of 19 genes, which were differentially expressed by clinical obesity indices in the PBMCs of high fat-fed rats, were determined in their PBMCs using real-time PCR.
The expression of 9 of 19 previously selected genes was significantly correlated with one or more clinical obesity indices. Both TFEC and CCL2 expression were negatively correlated with BMI, fat amount, fat percentage, waist/hip ratio, and leptin concentration. Similarly, TNFAIP2, VCAN, ASSI, IRF1, and HK3 expression negatively correlated with some clinical obesity indices, such as TNFAIP2 for BMI, fat amount, fat percentage, and waist/hip ratio, VCAN for fat amount, fat percentage, and waist/hip ratio, ASS1 for BMI and fat amount, IRF1 for BMI, fat amount, and fat percentage, and HK3 for fat amount. In contrast, both TNF-α and LPL expression were positively correlated with waist/hip ratio.
We identified 9 of 19 genes in human PBMCs that significantly correlated with one or more clinical obesity indices. Because these genes have a mechanistic basis for the development or progression of obesity and its metabolic derangements, they may help to determine possible underlying mechanisms for obesity.
人类外周血单核细胞（PBMC）中的基因表达谱可能是更好地理解肥胖的有用工具。我们研究了 PBMC 中的基因表达水平，以寻找肥胖和非肥胖受试者（19-55 岁）之间的可能差异，并评估了 PBMC 中基因表达与临床肥胖指数之间的相关性。
在 30 名肥胖和 20 名非肥胖受试者中测定体重、BMI、脂肪量、脂肪百分比、腰臀比、瘦素和脂联素水平。使用实时 PCR 测定其 PBMC 中 19 个基因的表达水平，这些基因在高脂肪喂养大鼠的 PBMC 中根据临床肥胖指数差异表达。
19 个先前选择的基因中有 9 个的表达与一个或多个临床肥胖指数显著相关。TFEC 和 CCL2 的表达与 BMI、脂肪量、脂肪百分比、腰臀比和瘦素浓度呈负相关。同样，TNFAIP2、VCAN、ASSI、IRF1 和 HK3 的表达与一些临床肥胖指数呈负相关，如 TNFAIP2 与 BMI、脂肪量、脂肪百分比和腰臀比，VCAN 与脂肪量、脂肪百分比和腰臀比，ASS1 与 BMI 和脂肪量，IRF1 与 BMI、脂肪量和脂肪百分比，HK3 与脂肪量。相比之下，TNF-α 和 LPL 的表达与腰臀比呈正相关。
我们在人类 PBMC 中鉴定了 19 个基因中的 9 个，它们与一个或多个临床肥胖指数显著相关。由于这些基因在肥胖及其代谢紊乱的发展或进展中有其机制基础，它们可能有助于确定肥胖的潜在机制。
