Zarrati Mitra, Salehi Eisa, Nourijelyani Keramat, Mofid Vahid, Zadeh Mohammad Javad Hossein, Najafi Forouzan, Ghaflati Zahra, Bidad Katayoon, Chamari Maryam, Karimi Mehrdad, Shidfar Farzad
a Department of Nutrition, School of Health , Tehran University of Medical Sciences , Tehran , IRAN.
J Am Coll Nutr. 2014;33(6):417-25. doi: 10.1080/07315724.2013.874937. Epub 2014 Jul 31.
The purpose of this study was to investigate whether probiotics had an effect on proinflammatory markers and cytokines in overweight and obese individuals and whether they could have synergistic effects with weight-loss diets.
A total of 75 healthy overweight and obese individuals completed this randomized doubled-blind controlled clinical trial. Participants were randomly assigned to groups consuming regular yogurt with a low-calorie diet (LCD, RLCD; n = 25) or receiving probiotic yogurt with LCD (PLCD; n = 25) or consuming probiotic yogurt without LCD (PWLCD; n = 25) for 8 weeks. The pribiotic regimen contained 200 g/day yogurt, enriched by Lactobacillus acidophilus La5, Bifidobacterium BB12, and Lactobacillus casei DN001 10(8) colony-forming units/g. Body fat percentage, high-sensitive C-reactive protein (hs-CRP), tumor necrosis factor-alpha (TNF-α), leptin, and mRNA levels of inflammation-related genes (TNF-α and RAR-related orphan receptor gamma [ROR-γt]) in peripheral blood mononuclear cells (PBMCs) were measured.
A reduction in body mass index (BMI), fat percentage, and leptin level was observed that was more obvious in groups who received the weight-loss diet with probiotic yogurt. Reduction in the gene expression of ROR-γt was significant in the PLCD group (p < 0.001). The expression of TNF-α did not change among all groups after intervention. The mean concentration of leptin was significantly decreased in all groups after the dietary intervention, but the mean changes in leptin level in the PLCD group was more prominent compared to the other two groups (-2.38, p < 0.001 [PLCD] vs -1.75, p = 0.002 [RLCD] and -0.55 ng/mL, p = 0.12 [PWLCD]). The reduction in serum levels of hs-CRP was more evident in the PWLCD group compared to the PLCD and RLCD groups after the 8-week intervention (-3.4, p = 0.03 vs -1.76, p < 0.001 and -2.98 pg/mL, p < 0.001, respectively).
Our results suggested that the weight-loss diet and probiotic yogurt had synergistic effects on T-cells subset specific gene expression in PBMCs, fat percentage, and body weight among overweight and obese individuals.
本研究旨在调查益生菌对超重和肥胖个体的促炎标志物和细胞因子是否有影响，以及它们是否能与减肥饮食产生协同作用。
共有75名健康的超重和肥胖个体完成了这项随机双盲对照临床试验。参与者被随机分配到三组，分别是食用含低热量饮食的普通酸奶组（低热量饮食组，RLCD；n = 25），或食用含低热量饮食的益生菌酸奶组（益生菌低热量饮食组，PLCD；n = 25），或食用不含低热量饮食的益生菌酸奶组（无低热量饮食的益生菌酸奶组，PWLCD；n = 25），为期8周。益生菌方案为每天食用200克酸奶，其中富含嗜酸乳杆菌La5、双歧杆菌BB12和干酪乳杆菌DN001，每克含10(8)个菌落形成单位。测量了体脂百分比、高敏C反应蛋白（hs-CRP）、肿瘤坏死因子-α（TNF-α）、瘦素以及外周血单个核细胞（PBMCs）中炎症相关基因（TNF-α和视黄酸相关孤儿受体γ [ROR-γt]）的mRNA水平。
观察到体重指数（BMI）、脂肪百分比和瘦素水平有所降低，在食用含益生菌酸奶的减肥饮食组中更为明显。PLCD组中ROR-γt的基因表达显著降低（p < 0.001）。干预后所有组中TNF-α的表达均未改变。饮食干预后所有组中瘦素的平均浓度均显著降低，但PLCD组中瘦素水平的平均变化比其他两组更为显著（-2.38，p < 0.001 [PLCD] 对比 -1.75，p = 0.002 [RLCD] 和 -0.55 ng/mL，p = 0.12 [PWLCD]）。8周干预后，PWLCD组中hs-CRP的血清水平降低比PLCD组和RLCD组更明显（分别为-3.4，p = 0.03对比 -1.76，p < 0.001和 -2.98 pg/mL，p < 0.001）。
我们的结果表明，减肥饮食和益生菌酸奶对超重和肥胖个体的PBMCs中T细胞亚群特异性基因表达、脂肪百分比和体重有协同作用。
