Department of Telecommunications and Teleinformatics, Wroclaw University of Science and Technology, 50-370 Wroclaw, Poland.
Sensors (Basel). 2020 Jun 11;20(11):3321. doi: 10.3390/s20113321.
Fluorescence anisotropy imaging and sensing is a widely recognized method for studying molecular orientation and mobility. However, introducing this technique to in vivo systems is a challenging task, especially when one considers multiphoton excitation methods. Past two decades have brought a possible solution to this issue in the form of hollow-core antiresonant fibers (HC-ARFs). The continuous development of their fabrication technology has resulted in the appearance of more and more sophisticated structures. One of the most promising concepts concerns dual hollow-core antiresonant fibers (DHC-ARFs), which can be used to split and combine optical signals, effectively working as optical fiber couplers. In this paper, the design of a fluorescence anisotropy sensor based on a DHC-ARF structure is presented. The main purpose of the proposed DHC-ARF is multiphoton-excited fluorescence spectroscopy; however, other applications are also possible.
荧光各向异性成像和传感是一种广泛认可的研究分子取向和迁移的方法。然而，将该技术引入活体系统是一项具有挑战性的任务，特别是当考虑多光子激发方法时。在过去的二十年中，空心反谐振光纤（HC-ARF）为解决这一问题提供了一种可能的解决方案。其制造技术的不断发展导致了越来越复杂的结构的出现。其中最有前途的概念之一涉及双空心反谐振光纤（DHC-ARF），它可用于分离和组合光学信号，有效地充当光纤耦合器。本文提出了一种基于 DHC-ARF 结构的荧光各向异性传感器的设计。所提出的 DHC-ARF 的主要目的是多光子激发荧光光谱学；然而，也可能有其他应用。
