National HIV and Retrovirology Laboratories at JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB R3E 3L5, Canada.
Scientific Informatics Services at National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada.
Viruses. 2020 Jun 14;12(6):645. doi: 10.3390/v12060645.
Next-generation sequencing (NGS) in HIV drug resistance (HIVDR) testing has the potential to improve both clinical and public health settings, however it challenges the normal operations of quality management systems to be more flexible due to its complexity, massive data generation, and rapidly evolving protocols. While guidelines for quality management in NGS data have previously been outlined, little guidance has been implemented for NGS-based HIVDR testing. This document summarizes quality control procedures for NGS-based HIVDR testing laboratories using a laboratory information systems (LIS) framework. Here, we focus in particular on the quality control measures applied on the final sequencing product aligned with the recommendations from the World Health Organization HIV Drug Resistance Laboratory Network.
下一代测序（NGS）在艾滋病病毒耐药性（HIVDR）检测中具有改善临床和公共卫生环境的潜力，但由于其复杂性、大量数据生成和快速发展的协议，对质量管理系统的正常运行提出了更高的灵活性要求。虽然之前已经概述了 NGS 数据质量管理指南，但针对基于 NGS 的 HIVDR 检测，实施的指导很少。本文采用实验室信息系统（LIS）框架，总结了基于 NGS 的 HIVDR 检测实验室的质量控制程序。在这里，我们特别关注与世界卫生组织 HIV 耐药性实验室网络的建议一致的最终测序产物的质量控制措施。
