Azuma M, Seki T, Fujishita S
Department of Health Science, Osaka Kyoiku University, Japan.
Vision Res. 1988;28(9):959-64. doi: 10.1016/0042-6989(88)90072-7.
Retinoids in the eyes of Xenopus laevis at several developmental stages, were analyzed by high-performance liquid chromatography (HPLC). At stage 37/38, larval eyes contained mainly all-trans isomers of retinal, 3-dehydroretinal, retinyl ester and 3-dehydroretinyl ester. Ratios of all-trans 3-dehydroretinal to retinal and of all-trans 3-dehydroretinyl ester to retinyl ester were almost 1 at the stage. With the advance of development, the amounts of all-trans retinal and 3-dehydroretinal decreased; however, those of all-trans retinyl ester and 3-dehydroretinyl ester increased. The chromophores of visual pigments, 11-cis retinal and 3-dehydroretinal, were detected at stage 40 (total; 0.2 pmol/eye) and their amounts increased after that stage. The ratio of 11-cis 3-dehydroretinal to retinal was almost 1 at stages 40-42. The ratio became larger after stage 43 and was almost 19 at stage 46. The ratio of all-trans 3-dehydroretinyl ester to retinyl ester, also, increased after stage 42 and reached 11 at stage 46. The mechanism of 11-cis formation during development is discussed in relation to retinoid conversions in the eyes.
利用高效液相色谱法（HPLC）分析了非洲爪蟾在几个发育阶段眼睛中的类视黄醇。在第37/38阶段，幼体眼睛主要含有视黄醛的全反式异构体、3 - 脱氢视黄醛、视黄酯和3 - 脱氢视黄酯。在该阶段，全反式3 - 脱氢视黄醛与视黄醛以及全反式3 - 脱氢视黄酯与视黄酯的比例几乎为1。随着发育进程，全反式视黄醛和3 - 脱氢视黄醛的量减少；然而，全反式视黄酯和3 - 脱氢视黄酯的量增加。在第40阶段检测到视觉色素的发色团11 - 顺式视黄醛和3 - 脱氢视黄醛（总量为0.2 pmol/眼），并且在该阶段之后其含量增加。在第40 - 42阶段，11 - 顺式3 - 脱氢视黄醛与视黄醛的比例几乎为1。在第43阶段之后该比例变大，在第46阶段几乎为19。全反式3 - 脱氢视黄酯与视黄酯的比例在第42阶段之后也增加，在第46阶段达到11。结合眼睛中的类视黄醇转化讨论了发育过程中11 - 顺式形成的机制。
